Biomarker: miRNA 27a

Histology type: endometrioid endometrial carcinoma

Region: Shatby Alexandria

Followed up time :

Subgroup 1 name : un-intervened endometrial cancer

Subgroup 1 number: 36

Subgroup 2 name: healthy controls

Subgroup 2 number: 36

Cohort statistics: Mean (SD);Range

Description: MiRNA 27a was significantly associated with type I endometroid endometrial cancer.

Sample type : serum

Sample method: RT-qPCR

Expression pattern : circulating overexpression cut off= 0.2872

Expression elevation: Relative quantifcation miRNA expression in patients ΔΔCt: is the diference of ΔCt value between the cases and the average of control (ΔΔCt=ΔCt cases – average ΔCt control). The relative quantifcation of miRNA expression was presented as the fold change normalized to the spike in reference mirna 39 and relative to the healthy control samples. Relative quantifcation miRNA expression in healthy control=2–ΔΔCt ΔCt = Ct (target miRNA) − Ct (reference miRNA) ΔΔCt = ΔCt of control − average ΔCt of control

Subgroup 1 age: 57.6 ± 8.3

Subgroup 2 age: 53.7±9.4

HGNC miRNA approved symbol: MIR27A

HGNC miRNA approved name: microRNA 27a

HGNC miRNA locus type: RNA, micro

HGNC miRNA symbol status: Approved

HGNC miRNA ID: HGNC:31613

HGNC miRNA link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:31613

Stem loop sequence symbol: hsa-mir-27a

Stem loop sequence accession: MI0000085

Stem loop sequence gene family: MIPF0000036

Stem loop sequence annotation confidence: high

Mature sequence symbol: hsa-miR-27a-5p $hsa-miR-27a-3p

Mature sequence accession: MIMAT0004501$ MIMAT0000084

Previous IDs: hsa-miR-27a*$ hsa-miR-27a

Evidence: experimental; cloned[PMID:17604727]$ experimental; cloned [PMID:11679670,PMID:15325244,PMID:17604727,PMID:17616659], Northern [PMID:11679670]

miRBase link: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000085