Basic information
Biomarker: lncRNA NEAT1
Histology type: endometrial carcinoma
Cohort characteristics
Country: China
Region: Guangxi
Followed up time :
| Total number | Group I | Group I number | Group II | Group II number | Group III | Group III number | Group IV | Group IV number |
|---|---|---|---|---|---|---|---|---|
| 60 | EC | 30 | normal endometrial | 30 |
Sample information
Description: Our study reveals that exosomal NEAT1 from CAFs contributes to EC progression via miR-26a/b-5p-mediated STAT3/YKL-40 pathway, which indicates the therapeutic potential of exosomal NEAT1 for treating EC.
Sample type : tissue
Sample method: Real-time quantitative PCR analysis,Western blot analysis
Expression pattern : exosomal
Validation in cell line : cell lines HEC-1A, HEC-1B, and RL95-2
Validation in cell method : Immunofluorescence,Real-time quantitative PCR analysis,Western blot analysis,Luciferase analysis,RIP assay，Chromatin immunoprecipitation (ChIP)，MTT assay，Colony formation assay，Tumorigenicity assay in vivo
Validation involved pathway : miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway
Disease information
Related information
Expression figure legend: Taken together, our study reveals that exosomal NEAT1 from CAFs contributes to EC progression via miR-26a/b-5p-mediated STAT3/YKL-40 pathway, which indicates the therapeutic potential of exosomal NEAT1 for treating EC.
Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233173/figure/fig0001/
Funtion description: NEAT1 and YKL-40 were up-regulated, while miR-26a/b-5p was down-regulated in EC tissues. Moreover, NEAT1 expression was increased in CAF-exosomes compared with that in NF-exosomes. In addition, the exosomal NEAT1 derived from CAFs could transfer to EC cells and promote YKL-40 expression. Further exploration showed that exosomal NEAT1 enhanced YKL-40 expression via regulating miR-26a/b-5p-STAT3 axis in EC cells. More importantly, exosomal NEAT1 accelerated in vivo tumor growth via miR-26a/b-5p-STAT3-YKL-40 axis.
