Basic information

Biomarker: lncRNA NEAT1

Histology type: endometrial carcinoma

Cohort characteristics

Country: China

Region: Guangxi

Followed up time :

Total number Group I Group I number Group II Group II number Group III Group III number Group IV Group IV number
60 EC 30 normal endometrial 30

Sample information

Description: Our study reveals that exosomal NEAT1 from CAFs contributes to EC progression via miR-26a/b-5p-mediated STAT3/YKL-40 pathway, which indicates the therapeutic potential of exosomal NEAT1 for treating EC.

Sample type : tissue

Sample method: Real-time quantitative PCR analysis,Western blot analysis

Expression pattern : exosomal

Validation in cell line : cell lines HEC-1A, HEC-1B, and RL95-2

Validation in cell method : Immunofluorescence,Real-time quantitative PCR analysis,Western blot analysis,Luciferase analysis,RIP assay,Chromatin immunoprecipitation (ChIP),MTT assay,Colony formation assay,Tumorigenicity assay in vivo

Validation involved pathway : miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway

Disease information

Related information

Expression figure legend: Taken together, our study reveals that exosomal NEAT1 from CAFs contributes to EC progression via miR-26a/b-5p-mediated STAT3/YKL-40 pathway, which indicates the therapeutic potential of exosomal NEAT1 for treating EC.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233173/figure/fig0001/

Funtion description: NEAT1 and YKL-40 were up-regulated, while miR-26a/b-5p was down-regulated in EC tissues. Moreover, NEAT1 expression was increased in CAF-exosomes compared with that in NF-exosomes. In addition, the exosomal NEAT1 derived from CAFs could transfer to EC cells and promote YKL-40 expression. Further exploration showed that exosomal NEAT1 enhanced YKL-40 expression via regulating miR-26a/b-5p-STAT3 axis in EC cells. More importantly, exosomal NEAT1 accelerated in vivo tumor growth via miR-26a/b-5p-STAT3-YKL-40 axis.

Visulization