Biomarker: microRNA-21

Histology type: endometrioid endometrial carcinoma

Stage: advanced stage

Country: Japan

Region: Saitama

Study type: Comparative Study

Followed up time :

Subgroup 1 name : stroma cell high expression

Subgroup 1 number: 161

Subgroup 2 name: stroma cell low expression

Subgroup 2 number: 69

Cohort statistics: cutoff<60;cutoff>60

Description: High miR-21 expression in cancer cells was an independent prognostic factor associated with poor progression-free survival, as well as older age and higher International Federation of Gynecology and Obstetrics (FIGO) stage.

Sample type : tissue

Sample method: in situ hybridization (ISH)/qRT‑PCR/ISH analysis/Immunohistochemistry

Expression pattern : high expression(mean TS≥4)

Expression elevation: The miR-21 signals were semi-quantitatively evaluated with the following intensity score (IS): 0, negative; 1, weak; 2, moderate; and 3, strong; and proportional score (PS): 0, none; 1, 1–10%; 2, 10–50%; and 3,>51%. The total score (TS) was calculated by adding IS and PS. We evaluated miR21 expression in cancer cells and stromal cells separately.

Cohort age: 111;119

Subgroup 1 age: 82;79

Subgroup 2 age: 29;40

Expression figure legend: Representative miR-21 ISH staining images in tissue microarray samples of endometrioid endometrial carcinoma (× 400)

Expression figure link: https://link.springer.com/article/10.1007/s00428-021-03171-w/figures/1

Survival figure legend: survival curves for all endometrial cancer patients with respect to high and low miR-21 expression in cancer cells.

Survival curve link: https://link.springer.com/article/10.1007/s00428-021-03171-w/figures/2

HGNC miRNA approved symbol: MIR21

HGNC miRNA approved name: microRNA 21

HGNC miRNA locus type: RNA, micro

HGNC miRNA symbol status: Approved

HGNC miRNA ID: HGNC:31586

HGNC miRNA link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:31586

Stem loop sequence symbol: hsa-mir-21

Stem loop sequence accession: MI0000077

Stem loop sequence gene family: MIPF0000060

Stem loop sequence annotation confidence: high

Mature sequence symbol: hsa-miR-21-5p$ hsa-miR-21-3p

Mature sequence accession: MIMAT0000076$ MIMAT0004494

Previous IDs: hsa-miR-21$ hsa-miR-21*

Evidence: experimental; cloned[PMID:11679670,PMID:11914277,PMID:14573789,PMID:15325244,PMID:15978578,PMID:17604727,PMID:17616659], Northern [11679670,PMID:15183728], Illumina [PMID:20158877]$ experimental; cloned [PMID:17604727,PMID:17616659]

miRBase link: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000077