Basic information
Biomarker: microRNA-21
Histology type: endometrioid endometrial carcinoma
Stage: advanced stage
Cohort characteristics
Country: Japan
Region: Saitama
Study type: Comparative Study
Followed up time :
Subgroup 1 name : cancer cell high expression
Subgroup 1 number: 42
Subgroup 2 name: cancer cell low expression
Subgroup 2 number: 188
Cohort statistics: cutoff<60;cutoff>60
Total number | Group I | Group I number | Group II | Group II number | Group III | Group III number | Group IV | Group IV number |
---|---|---|---|---|---|---|---|---|
460 | cancer cell | 230 | stroma cell | 230 |
Sample information
Description: High miR-21 expression in cancer cells was an independent prognostic factor associated with poor progression-free survival, as well as older age and higher International Federation of Gynecology and Obstetrics (FIGO) stage.
Sample type : tissue
Sample method: in situ hybridization (ISH)/qRT‑PCR/ISH analysis/Immunohistochemistry
Expression pattern : high expression(mean TS≥4)
Expression elevation: The miR-21 signals were semi-quantitatively evaluated with the following intensity score (IS): 0, negative; 1, weak; 2, moderate; and 3, strong; and proportional score (PS): 0, none; 1, 1–10%; 2, 10–50%; and 3,>51%. The total score (TS) was calculated by adding IS and PS. We evaluated miR21 expression in cancer cells and stromal cells separately.
Disease information
Cohort age: 111;119
Subgroup 1 age: 16;26
Subgroup 2 age: 95;93
Related information
Expression figure legend: Representative miR-21 ISH staining images in tissue microarray samples of endometrioid endometrial carcinoma (× 400)
Expression figure link: https://link.springer.com/article/10.1007/s00428-021-03171-w/figures/1
Survival figure legend: survival curves for all endometrial cancer patients with respect to high and low miR-21 expression in cancer cells.
Survival curve link: https://link.springer.com/article/10.1007/s00428-021-03171-w/figures/2
HGNC miRNA approved symbol: MIR21
HGNC miRNA approved name: microRNA 21
HGNC miRNA locus type: RNA, micro
HGNC miRNA symbol status: Approved
HGNC miRNA ID: HGNC:31586
HGNC miRNA link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:31586
Stem loop sequence symbol: hsa-mir-21
Stem loop sequence accession: MI0000077
Stem loop sequence gene family: MIPF0000060
Stem loop sequence annotation confidence: high
Mature sequence symbol: hsa-miR-21-5p$ hsa-miR-21-3p
Mature sequence accession: MIMAT0000076$ MIMAT0004494
Previous IDs: hsa-miR-21$ hsa-miR-21*
Evidence: experimental; cloned[PMID:11679670,PMID:11914277,PMID:14573789,PMID:15325244,PMID:15978578,PMID:17604727,PMID:17616659], Northern [11679670,PMID:15183728], Illumina [PMID:20158877]$ experimental; cloned [PMID:17604727,PMID:17616659]
miRBase link: https://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000077