Biomarker: PKN1

Histology type: endometrial cancer

Stage: high-grade

Country: Sweden

Region: Stockholm

Followed up time :

Conclusion: We have shown a trend of the higher PKN1 expression levels in EC patients with poor prognosis. Therefore, PKN1 might be considered as a candidate prognostic marker for EC.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : upregulated

Statictics: Median(25–75 Percentile)

Cohort age: 71.0 (67.0–77.0)

Funtion Uniprot: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro

Biological function from UniProt: #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.

Subcellular UniProt: #Cell membrane #Cytoplasm #Endosome #Membrane #Nucleus

Alternative name from UniProt:

Catalytic activity: ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]

Activity regulation: Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.

Recommended name: Serine/threonine-protein kinase N1

Gene name from HGNC: PKN1

AlphaFold DB: Q16512

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q16512

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Heart muscle - Cardiac muscle contraction (mainly)

Single cell type specificity Cell type enhanced (monocytes)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Localized to the Plasma membrane In addition localized to the Cytokinetic bridge

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000123143-PKN1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000123143-PKN1/pathology/endometrial+cancer

Expression figure legend: Expression pattern of the PKN1 protein in EC samples.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092572/figure/fig2-10732748221094797/

Survival figure legend: PKN1 expression levels correlate with a survival rate.

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092572/figure/fig4-10732748221094797/

OMIM: 601032

OMIM link2: https://www.omim.org/entry/601032