Biomarker: B7-H3

Histology type: endometrial serous carcinomas

Country: USA

Region: TX

Followed up time :

Subgroup 1 name : positive

Subgroup 1 number: 91

Subgroup 2 name: negative

Subgroup 2 number: 7

Conclusion: In conclusion, PD-L1, TIM-3 and B7-H3 may be potential therapeutic targets in selected patients with ESC. Further investigation of their roles as predictive biomarkers is needed.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: For all three immune checkpoint markers, cutoff scores of ≥1% for TPS and ≥1 for CPS were used to define positivity, and additional cutoffs (10, 20, and 50) were used to further analyze the significance of immune checkpoint expression. Due to the lack of established criteria for assessment of TIM-3 and B7-H3, the same scoring systems and positivity thresholds used for PD-L1 assessment were applied.

Statictics: Median;Range

Cohort age: 64 (49–83)

Funtion Uniprot: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

UniProt ID: Q5ZPR3

UniProt Link: https://www.uniprot.org/uniprotkb/Q5ZPR3/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Subcellular UniProt: #Membrane

Alternative name from UniProt:

Miscellaneous: B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

Recommended name: CD276 antigen

Gene name from HGNC: CD276 (B7-H3, B7H3, B7RP-2)

CD antigen name: CD276

HPA class: CD markers

AlphaFold DB: Q5ZPR3

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q5ZPR3

Induction: By bacterial lipopolysaccharides (LPS) in monocytes and by ionomycin in T and B-lymphocytes. Up-regulated in cells mediating rejection of human transplants.

HPA link: https://www.proteinatlas.org/ENSG00000103855-CD276

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic and membranous expression.

Single cell type specificity Cell type enhanced (Extravillous trophoblasts, Hofbauer cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Localized to the Vesicles

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000103855-CD276/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000103855-CD276/pathology/endometrial+cancer

Survival figure legend: Kaplan–Meier curves of progression-free survival (PFS) and overall survival (OS) and correlation with TIM-3 and B7-H3 expression.

Survival curve link: https://ars.els-cdn.com/content/image/1-s2.0-S0893395222055077-gr6.jpg

OMIM: 605715

OMIM link2: https://www.omim.org/entry/605715

HGNC ID: HGNC:19137

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:19137