Biomarker: CK-20

Histology type: endometrial carcinoma

Country: Israel

Region: Kfar Saba

Followed up time :

Subgroup 1 name : positive

Subgroup 1 number: 17

Subgroup 2 name: negative

Subgroup 2 number: 1

Conclusion: These results indicate that RT-PCR of CK-20, because of its high sensitivity, is a potential biomarker for detecting endometrial carcinoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : positive expression

Expression elevation: The results of immunohistochemical staining were evaluated and scored according to the consensus report of the EORTC-GCCG

Funtion Uniprot: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity)

UniProt ID: P35900

UniProt Link: https://www.uniprot.org/uniprotkb/P35900/entry

Biological function from UniProt: #Apoptosis

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa

Subcellular UniProt: #Cytoplasm #Intermediate filament #Keratin

Alternative name from UniProt:

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Recommended name: Keratin, type I cytoskeletal 20

Gene name from HGNC: KRT20 (CK20, K20, MGC35423)

HPA class: Plasma proteins

AlphaFold DB: P35900

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P35900

Developmental stage: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.

HPA link: https://www.proteinatlas.org/ENSG00000171431-KRT20

Tissue specificity RNA from HPA: Tissue enriched (intestine)

Tissue expression from HPA: Selective cytoplasmic expression in gastrointestinal epithelium.

Single cell type specificity Group enriched (Paneth cells, Distal enterocytes, Proximal enterocytes, Intestinal goblet cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Cytosol

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000171431-KRT20/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000171431-KRT20/pathology/endometrial+cancer

OMIM: 608218

OMIM link2: https://www.omim.org/entry/608218

HGNC ID: HGNC:20412

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:20412