Biomarker: MCM5

Histology type: endometrial carcinoma

Country: English

Region: Manchester

Followed up time :

Subgroup 1 name : Endometrial cancer

Subgroup 1 number: 41

Subgroup 2 name: Ovarian cancer

Subgroup 2 number: 26

Conclusion: MCM5 is a novel sensitive and specific biomarker for the detection of ovarian and endometrial tumours in urine samples, which is likely to have clinical utility as a diagnostic aid.

Sample type : urine

Sample method: ELISA

Expression pattern : cut-off = 12 pg/mL

Expression elevation: Concentrations of MCM5 were calculated using a serial dilution standard curve of a known recombinant MCM5 control (1.3 mg/ml) with a negative control

Statictics: Mean (SD);Range

Subgroup 1 age: 70;57-77

Subgroup 2 age: 63;52-69

Funtion Uniprot: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

UniProt ID: P33992

UniProt Link: https://www.uniprot.org/uniprotkb/P33992/entry

Biological function from UniProt: Cell cycle, DNA replication

Molecular function from UniProt:

Subcellular UniProt: Cellular component #Chromosome #Cytoplasm #Nucleus

Alternative name from UniProt:

Miscellaneous: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.

Recommended name: DNA replication licensing factor MCM5

Gene name from HGNC: MCM5 (CDC46)

HPA class: Cancer-related genes Disease related genes Enzymes Human disease related genes Plasma proteins Potential drug targets

AlphaFold DB: P33992

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P33992

HPA link: https://www.proteinatlas.org/ENSG00000100297-MCM5

Tissue specificity RNA from HPA: Tissue enhanced (bone marrow)

Tissue expression from HPA: Nuclear expression mainly in hematopoietic cells, immune cells, cytotrophoblastic cells in placenta and cells in testis. Also expressed in other cell types.

Single cell type specificity Cell type enhanced (Cytotrophoblasts, monocytes, Erythroid cells, Syncytiotrophoblasts)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm (Single cell variability, CCD Protein, CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in cervical cancer (favorable), renal cancer (unfavorable), liver cancer (unfavorable) and lung cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000100297-MCM5/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000100297-MCM5/pathology/endometrial+cancer

Phenotype ID: 617564

Disease: Meier-Gorlin syndrome 8 (MGORS8)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 602696;617564;

OMIM link1: https://www.omim.org/entry/617564

OMIM link2: https://www.omim.org/entry/602696

HGNC ID: HGNC:6948

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:6948