Biomarker: p16

Histology type: endometrial carcinoma

Country: Finland

Region: Helsinki

Study type: retrospective study

Followed up time :

Subgroup 1 name : NSMP

Subgroup 1 number: 206

Subgroup 2 name: MMR-D

Subgroup 2 number: 264

Conclusion: P16 hyperexpression appear to have a stronger prognostic impact in NSMP（no specific molecular profile ）as compared to MMR-D EC.

Sample type : tissue

Sample method: immunohistochemistry/RT-PCR

Expression pattern : hyper expression

Expression elevation: As negative p16 was extremely rare (1.7% of the samples), only block type hyperexression of p16 was classified as abnormal staining.

Funtion Uniprot: Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

UniProt ID: Q8N726

UniProt Link: https://www.uniprot.org/uniprotkb/Q8N726/entry#function

Biological function from UniProt: Apoptosis, Cell cycle, rRNA processing, Transcription, Transcription regulation, Ubl conjugation pathway

Molecular function from UniProt:

Subcellular UniProt: #Mitochondrion #Nucleus

Alternative name from UniProt:

Caution: The proteins described here are encoded by the gene CDKN2A, but are completely unrelated in terms of sequence and function to cyclin-dependent kinase inhibitor 2A (AC P42771) which is encoded by the same gene.

Recommended name: Tumor suppressor ARF

Gene name from HGNC: CDKN2A (ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, p14, p14ARF, p16, p16INK4a, p19, p19Arf)

HPA class: Cancer-related genes Disease related genes Human disease related genes Plasma proteins

AlphaFold DB: Q8N726

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q8N726

HPA link: https://www.proteinatlas.org/ENSG00000147889-CDKN2A

Tissue specificity RNA from HPA: Tissue enhanced (choroid plexus, pituitary gland)

Tissue expression from HPA: Mainly nuclear expression in most tissues.

Single cell type specificity Cell type enhanced (Late spermatids, Pancreatic endocrine cells, Early spermatids, Respiratory ciliated cells, Basal respiratory cells, Club cells)

Immune cell specificity: Immune cell enhanced (T-reg)

Subcellular summary HPA Located in Nucleoli (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in endometrial cancer (unfavorable), renal cancer (unfavorable), liver cancer (unfavorable) and head and neck cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000147889-CDKN2A/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000147889-CDKN2A/pathology/endometrial+cancer

Survival curve link: https://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0253472.g002

OMIM: 600160

OMIM link2: https://www.omim.org/entry/600160

HGNC ID: HGNC:1787

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1787