Biomarker: CD10

Histology type: endometrial stromal tumor

Country: Northern Ireland

Region: Belfast

Followed up time :

Subgroup 1 name : positive in endometrial stromal neoplasms

Subgroup 2 name: nagtive in endometrial stromal neoplasms

Subgroup 2 number: 30

Conclusion: CD10 is a reliable and sensitive immunohistochemical marker of normal endometrial stroma. Positivity, which is often strong and/or diffuse is found in endometrial stromal nodules and low-grade ESS. Positive staining with CD10, when strong and diffuse, may be useful in distinguishing these tumours from histological mimics, especially cellular leiomyoma and AGCT which are generally negative. In this situation, CD10 should be used as part of a panel which might include desmin and a-inhibin depending on the differential diagnosis considered. Positive staining with CD10 in a high-grade uterine sarcoma which is negative with muscle markers might indicate endometrial stromal differentiation and identify a group of neoplasms which it is correct to diagnose as high-grade ESS rather than undifferentiated uterine sarcoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression elevation: Positive staining was subjectively classied as weak, moderate or strong. Staining was further classied as focal or diffuse (arbitrarily taken as involving >50% of the tumour).

Subgroup 2 age: 78

Funtion Uniprot: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:6349683, PubMed:6208535, PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:6349683, PubMed:17101991). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:6349683, PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:2531377, PubMed:2972276, PubMed:16254193). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

UniProt ID: P08473

UniProt Link: https://www.uniprot.org/uniprotkb/P08473/entry

Molecular function from UniProt:

Subcellular UniProt: #Cell membrane #Membrane

Alternative name from UniProt:

Miscellaneous: Important cell surface marker in the diagnostic of human acute lymphocytic leukemia

Catalytic activity: Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.H2O + substance P = L-Leu-L-Met-NH2 + substance P(1-9)This reaction proceeds in the forward direction.;H2O + substance P = L-Phe-Gly-L-Leu-L-Met-NH2 + substance P(1-7) This reaction proceeds in the forward direction;H2O + neurotensin = L-isoleucyl-L-leucine + neurotensin(1-11) This reaction proceeds in the forward direction.;H2O + neurotensin = L-tyrosyl-L-isoleucyl-L-leucine + neurotensin(1-10) This reaction proceeds in the forward direction.;

Activity regulation: Inhibited in a dose dependent manner by opiorphin (PubMed:17101991). Activated by K49-P1-20, a twenty-residue synthetic peptide shortened from the snake B.asper myotoxin II (PubMed:26931059).

Recommended name: Neprilysin

Gene name from HGNC: MME (CALLA, CD10, NEP)

CD antigen name: CD10

HPA class: Cancer-related genes Candidate cardiovascular disease genes CD markers Disease related genes Enzymes FDA approved drug targets Human disease related genes Plasma proteins

AlphaFold DB: P08473

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P08473

HPA link: https://www.proteinatlas.org/ENSG00000196549-MME

Tissue specificity RNA from HPA: Tissue enhanced (intestine, kidney)

Tissue expression from HPA: Distinct luminal membrane expression in the small intestine, kidney, epididymis and prostate. Membranous expression in hepatocytes.

Single cell type specificity Cell type enhanced (Proximal enterocytes, Endometrial stromal cells, Syncytiotrophoblasts, Proximal tubular cells)

Immune cell specificity: Immune cell enriched (neutrophil)

Cancer prognostic summary HPA Group enriched (basal ganglia, medulla oblongata, pons, thalamus)

Pathology link: https://www.proteinatlas.org/ENSG00000196549-MME/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000196549-MME/pathology/endometrial+cancer

Phenotype ID: 617017;617018;

Disease: Charcot-Marie-Tooth disease 2T (CMT2T);Spinocerebellar ataxia 43 (SCA43);

Note1: The disease is caused by variants affecting the gene represented in this entry;The disease is caused by variants affecting the gene represented in this entry;

OMIM: 120520;617017;617018;

OMIM link1: https://www.omim.org/entry/617017;https://www.omim.org/entry/617018;

OMIM link2: https://www.omim.org/entry/120520;https://www.omim.org/entry/617017;https://www.omim.org/entry/617018

HGNC ID: HGNC:7154

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7154