Biomarker: fatty acid synthase (FAS)

Histology type: endometrial carcinoma

Stage: high risk

Country: Italy

Region: Rome

Followed up time :

Subgroup 1 name : positive

Subgroup 1 number: 53

Subgroup 2 name: negative

Subgroup 2 number: 33

Conclusion: FAS is a reliable marker of clinically aggressive endometrial carcinomas. The knowledge of FAS expression in endometrial carcinomas is an important finding that may stratify patients into selected groups and determine therapeutic approaches for patient care.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: semiquantitative method

Statictics: Median;Range

Cohort age: 65; 29–92

Funtion Uniprot: Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.

UniProt ID: P49327

UniProt Link: https://www.uniprot.org/uniprotkb/P49327/entry

Biological function from UniProt: #Fatty acid biosynthesis #Fatty acid metabolism #Lipid biosynthesis #Lipid metabolism

Molecular function from UniProt:

Tissue specificity from UniProt: Ubiquitous. Prominent expression in brain, lung, liver and mammary gland.

Subcellular UniProt: #Cytoplasm

Alternative name from UniProt:

Miscellaneous: The relatively low beta-ketoacyl synthase activity may be attributable to the low 4'-phosphopantetheine content of the protein.

Catalytic activity: https://www.uniprot.org/uniprotkb/P49327/entry

Activity regulation: Activated by S-nitrosylation which promotes enzyme dimerization (PubMed:26851298). Cerulenin, a potent non-competitive pharmacological inhibitor of FAS, binds covalently to the active site of the condensing enzyme region, inactivating a key enzyme step in fatty acid synthesis (PubMed:16969344).

Recommended name: Fatty acid synthase

Gene name from HGNC: FASN (FAS, SDR27X1)

HPA class: Cancer-related genes Enzymes FDA approved drug targets Metabolic proteins Plasma proteins

AlphaFold DB: P49327

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P49327

HPA link: https://www.proteinatlas.org/ENSG00000169710-FASN

Tissue specificity RNA from HPA: Tissue enhanced (adipose tissue, breast)

Tissue expression from HPA: Cytoplasmic expression in adipocytes and glandular cells of breast.

Single cell type specificity Cell type enhanced (Breast glandular cells, Hepatocytes, Breast myoepithelial cells, Alveolar cells type 2)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Plasma membrane, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in cervical cancer (unfavorable) and renal cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000169710-FASN/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000169710-FASN/pathology/endometrial+cancer

OMIM: 600212

OMIM link2: https://www.omim.org/entry/600212

HGNC ID: HGNC:3594

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3594