Biomarker: MCM7

Histology type: endometrial carcinoma

Country: China

Region: Zhengzhou

Study type: retrospective study

Followed up time :

Subgroup 1 name : high labelling indices (%)

Subgroup 1 number: 103

Subgroup 2 name: low labelling indices (%)

Subgroup 2 number: 109

Conclusion: In this TMA study on endometrial carcinoma, MCM7 was found to be a more reliable and useful marker than Ki67 in assessing tumour proliferation and in the prognosis of patients.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : high labelling indices

Expression elevation: In general, 200–500 cells were assessed in each core. Two cores were evaluated for each tumour specimen and the staining indices in two cores of each tumour were usually similar. The average of the labelling indices of the two tissue cores was taken as the index for the tumour. The growth fraction of the tumours (percentage of tumour cells positive for MCM7 and Ki67) was classified into four groups: 1 (< 25%), 2 (25–50%), 3 (51–75%) and 4 (> 75%). For statistical analysis, those with a low (group 1) or high (groups 2–4) labelling index were compared.

Statictics: cutoff<45;cutoff>45

Cohort age: 44;168

Subgroup 1 age: 15;107

Subgroup 2 age: 29;59

Funtion Uniprot: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

UniProt ID: P33993

UniProt Link: https://www.uniprot.org/uniprotkb/P33993/entry

Biological function from UniProt: #Cell cycle #DNA replication

Molecular function from UniProt:

Subcellular UniProt: #Chromosome #Nucleus

Alternative name from UniProt:

Miscellaneous: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activity: ATP + H2O = ADP + H+ + phosphate,This reaction proceeds in the forward direction.

Recommended name: DNA replication licensing factor MCM7

Gene name from HGNC: MCM7 (CDC47, MCM2, PPP1R104)

HPA class: Cancer-related genes Enzymes Plasma proteins

AlphaFold DB: P33993

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P33993

HPA link: https://www.proteinatlas.org/ENSG00000166508-MCM7

Tissue specificity RNA from HPA: Tissue enhanced (bone marrow, lymphoid tissue)

Tissue expression from HPA: Nuclear expression in highly proliferative cells.

Single cell type specificity Cell type enhanced (Erythroid cells, Cytotrophoblasts

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm (CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in cervical cancer (favorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000166508-MCM7/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000166508-MCM7/pathology/endometrial+cancer

OMIM: 600592

OMIM link2: https://www.omim.org/entry/600592

HGNC ID: HGNC:6950

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:6950