Biomarker: c-FLIP

Histology type: endometrial carcinoma

Country: China

Region: Wuhan

Followed up time :

Subgroup 1 name : EC

Subgroup 1 number: 42

Subgroup 2 name: Normal

Subgroup 2 number: 22

Conclusion: It is concluded that c-FLIP might contribute to the carcinogenesis and aggressiveness of endometrial carcinoma and might be a useful prognostic factor in the tumor.

Sample type : tissue

Sample method: RT-PCR/Immunohistochemistry

Expression pattern : expression

Expression elevation: A mean percentage of positive tumor cells was determined in at least five areas randomly at high magnification (3400) and assigned to one of five categories: (a) 0, ,5%; (b) 1, 5–25%; (c) 2, 25–50%; (d) 3, 50–75%; and (e) 4, >75%. The intensity of c-FLIP immunostaining was scored as: (a) 1, weak; (b) 2, moderate; (c) 3, intense.For tumor showing heterogeneous staining, the predominant pattern was taken into account for scoring. The percentage of positive tumor cells and staining intensity were multiplied to produce a weighted score for each case. Cases with weighted score ,1 were defined as negative expression, otherwise were defined as positive. The percentage of immunoreactive cells was calculated to determine the PCNA-LI by counting a total of at least 1000 tumor cells at high magnification (3400).

Statictics: Average

Cohort age: 45 ± 3

Subgroup 1 age: 41 ± 4

Funtion Uniprot: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

UniProt ID: O15519

UniProt Link: https://www.uniprot.org/uniprotkb/O15519/entry

Biological function from UniProt: #Apoptosis #Host-virus interaction

Molecular function from UniProt:

Tissue specificity from UniProt: Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

Alternative name from UniProt:

Recommended name: CASP8 and FADD-like apoptosis regulator

Gene name from HGNC: CFLAR (c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT)

HPA class: Cancer-related genes Enzymes

AlphaFold DB: O15519

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O15519

Induction: Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle

HPA link: https://www.proteinatlas.org/ENSG00000003402-CFLAR

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic expression.

Single cell type specificity Cell type enhanced (Langerhans cells, Urothelial cells)

Immune cell specificity: Immune cell enriched (neutrophil)

Subcellular summary HPA Located in Nucleoplasm, Nuclear bodies, Plasma membrane, Cytosol (CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in head and neck cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000003402-CFLAR/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000003402-CFLAR/pathology/endometrial+cancer

OMIM: 603599

OMIM link2: https://www.omim.org/entry/603599

HGNC ID: HGNC:1876

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1876