Biomarker: CRBP-1

Histology type: endometrial carcinoma

Country: Italy

Region: Rome

Followed up time :

Conclusion: Our results indicate that CRBP-1 immunodetection may constitute an additional tool for histological grading of endometrial carcinoma. The CRBP-1 loss during the progression of endometrial cancer suggests a new potential target for pharmacological strategies aimed to counteract its progression by increased intracellular retinol bioavailability.

Sample type : tissue

Sample method: RT-PCR/immunohistochemistry

Expression pattern : loss

Expression elevation: Semiquantitative CRBP-1, ER and PR immunoreaction was estimated at × 200 magnification in at least 10 fields by two the authors, who used a grading system in arbitrary units as follows: absent (0), low and focal (0.5), positive (weakly positive, 1+; moderately positive, 2+; strongly positive, 3+).24 The interobserver reproducibility was >95%. For each case, the ratio of the score with the number of fields analyzed was calculated. Results were analyzed by means of Student's t-test. The differences were considered statistically significant for value of P<0.05.

Statictics: Mean

Cohort age: 61

Funtion Uniprot: Cytoplasmic retinol-binding protein (PubMed:22665496, PubMed:26900151, PubMed:28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed:15632377, PubMed:22665496).

UniProt ID: P09455

UniProt Link: https://www.uniprot.org/uniprotkb/P09455/entry

Biological function from UniProt: #Transport

Molecular function from UniProt:

Tissue specificity from UniProt: Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.

Subcellular UniProt: #Cytoplasm #Lipid droplet

Alternative name from UniProt:

Recommended name: Retinol-binding protein 1

Gene name from HGNC: RBP1 (CRABP-I, CRBP, CRBP1, CRBPI, RBPC)

HPA class: Metabolic proteins

AlphaFold DB: P09455

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P09455

HPA link: https://www.proteinatlas.org/ENSG00000114115-RBP1

Tissue specificity RNA from HPA: Tissue enhanced (ovary, pancreas)

Tissue expression from HPA: Cytoplasmic expression in several tissues, most abundant in Leydig cells and ovarian stromal cells.

Single cell type specificity Group enriched (Granulosa cells, Theca cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Nucleoplasm, Cytosol

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000114115-RBP1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000114115-RBP1/pathology/endometrial+cancer

Expression figure legend: Immunohistochemical detection of CRBP-1 expression in normal and neoplastic endometrial cells.$ In poorly differentiated areas (*) of an otherwise G2 endometrioid carcinoma, CRBP-1 expression is markedly reduced,

Expression figure link: https://www.nature.com/articles/3800586/figures/1$ https://www.nature.com/articles/3800586/figures/2

OMIM: 180260

OMIM link2: https://www.omim.org/entry/180260

HGNC ID: HGNC:9919

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:9919