Biomarker: HER-2

Histology type: endometrial carcinoma

Stage: advanced stage

Country: USA

Region: Ohio State

Followed up time :

Subgroup 1 name : positive FISH

Subgroup 1 number: 31

Subgroup 2 name: negative FISH

Subgroup 2 number: 382

Conclusion: Our results would suggest that HER-2 is an important oncogene in high grade and stage endometrial cancer, but plays only a minor role in the much more common low grade and stage tumors that encompass the majority of clinical practice.

Sample type : tissue

Sample method: tissue microarray/Immunohistochemistry and Fluorescent In Situ Hybridization

Expression pattern : expression and amplification

Expression elevation: Negative results were recorded for cases meeting one of the three following criteria: no staining, score _x005f_x0001_ 0; staining but without a membranous pattern, score= 0; or incomplete membranous staining or complete membranous staining in less than 10% of the tumor cells, score= 1. Positive results were recorded for cases meeting one of the two following criteria: complete membranous staining in greater than 10% of the tumor cells of moderate intensity, score=2, or completemembranous stainingin greater than 10% of the tumor cells of strong intensity, score = 3. Tumors cells displaying at least two centromeric chromosome 17 signals and multiple HER-2 signals, with a HER-2/CEP17 ratio
2.2 were considered consistent with amplification of HER-2. Tumors cells displaying at least two centromeric chromosome 17 signals and an equal number of HER-2 signals, with a HER-2/CEP17 ratio less than 2.2 were considered consistent with no amplification of HER-2.

Statictics: Median;Range

Cohort age: 62;17-89

Funtion Uniprot: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.Curated In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.3 Publications

UniProt ID: P04626

UniProt Link: https://www.uniprot.org/uniprotkb/P04626/entry

Biological function from UniProt: Transcription, Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth

Subcellular UniProt: #Cell membrane #Cytoplasm #Endosome #Membrane #Nucleus

Alternative name from UniProt:

Catalytic activity: ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]

Activity regulation: Activated by dimerization. Not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.

Gene name from HGNC: ERBB2 (CD340, HER-2, HER2, NEU, NGL)

CD antigen name: CD340

HPA link: https://www.proteinatlas.org/ENSG00000141736-ERBB2

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Cytoplasmic and membranous expression in most tissues.

Subcellular summary HPA Located in Plasma membrane, Cytosol

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable), endometrial cancer (unfavorable) and pancreatic cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000163507-CIP2A/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000163507-CIP2A/pathology/endometrial+cancer

OMIM: 137800;164870;167000;

OMIM link2: https://www.omim.org/entry/137800;https://www.omim.org/entry/164870;https://www.omim.org/entry/167000;

Phenotype: GLIOMA SUSCEPTIBILITY 1; GLM1;ERB-B2 RECEPTOR TYROSINE KINASE 2; ERBB2;

HGNC ID: HGNC:3430

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3430