Biomarker: KLK8

Histology type: endometrial carcinoma

Stage: early stage in menopausal women.

Country: Japan

Region: Fukuyama

Followed up time :

Subgroup 1 name : low expression

Subgroup 1 number: 17

Subgroup 2 name: overexpression

Subgroup 2 number: 27

Conclusion: We suggest that expression of TADG-14/KLK8may be regulated by sex steroid hormones in endometria. Our results indicate that elevated TADG-14/KLK8 expression is an early event in endometrial carcinogenesis;may potentially serve as a useful early biomarker for the detection of endometrial carcinomas in menopausal women.

Sample type : tissue

Sample method: Semiquantitative RT-PCR,immunohistochemistry

Expression pattern : expression

Expression elevation: Each tumor section was given a score obtained by multiplying the staining intensity (no staining = 0, weak staining = 1, and strong staining = 2) by the percentage of stained cells (0% = 0, 1–24% = 1, and 6 25% = 2). With this system, the maximum score was 4 and the minimum was 0 (negative staining). Weakly positive immunostaining was defi ned as a score of 1 or 2, while strongly positive immunostaining was defi ned as a score of 4.

Statictics: cutoff<60;cutoff>60

Cohort age: 27;17

Subgroup 1 age: 9;8

Subgroup 2 age: 18;9

Funtion Uniprot: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.

UniProt ID: O60259

UniProt Link: https://www.uniprot.org/uniprotkb/O60259/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes

Subcellular UniProt: #Cytoplasm #Secreted

Alternative name from UniProt:

Miscellaneous: Expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer.pH Dependence Optimum pH is 8.5. Active from pH 7-10

Catalytic activity: Cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys.

Activity regulation: Inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.

Recommended name: Kallikrein-8

Gene name from HGNC: KLK8 (HNP, neuropsin, ovasin, PRSS19, TADG14)

HPA class: Cancer-related genes Enzymes Plasma proteins

AlphaFold DB: O60259

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O60259

HPA link: https://www.proteinatlas.org/ENSG00000129455-KLK8

Tissue specificity RNA from HPA: Tissue enhanced (esophagus, skin, vagina)

Tissue expression from HPA: Selective expression in squamous epithelium.

Single cell type specificity Group enriched (Suprabasal keratinocytes, Basal keratinocytes)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Vesicles

Cancer prognostic summary HPA Prognostic marker in pancreatic cancer (unfavorable), endometrial cancer (unfavorable), lung cancer (unfavorable) and urothelial cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000129455-KLK8/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000129455-KLK8/pathology/endometrial+cancer

OMIM: 605644

OMIM link2: https://www.omim.org/entry/605644

HGNC ID: HGNC:6369

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:6369