Biomarker: Nm23

Histology type: endometrial carcinoma

Country: Turkey

Region: Bursa

Followed up time :

Subgroup 1 name : positive in lymph node involvement group

Subgroup 1 number: 15

Subgroup 2 name: positive in without lymph node involvement

Subgroup 2 number: 22

Conclusion: Elevated Nm23 expression is related to lower rates of lymph node metastasis and longer survival.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : elevated

Expression elevation: The slides were evaluated semiquantitatively according to their degree of cytoplasmic staining as follows: () for no staining or focal staining less than 25%; (+) for 26% to 50% staining; (++) for 51% to 75% staining; and (+++) for more than 76% staining.

Funtion Uniprot: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair

UniProt ID: P15531

UniProt Link: https://www.uniprot.org/uniprotkb/P15531/entry

Biological function from UniProt: #Differentiation #Endocytosis #Neurogenesis #Nucleotide metabolism

Molecular function from UniProt:

Tissue specificity from UniProt: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.

Subcellular UniProt: #Cytoplasm #Nucleus

Alternative name from UniProt:

Miscellaneous: The role of this protein in tumor development and progression is uncertain. This protein is found in reduced amount in some tumor cells of high metastatic potential. However, increased NME1 levels correlate with aggressive tumor features in neuroblastoma. May have distinct if not opposite roles in different tumors.

Activity regulation: Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits the endonuclease activity.

Recommended name: Nucleoside diphosphate kinase A

Gene name from HGNC: NME1 (NDPKA, NM23, NM23-H1)

HPA class: Cancer-related genes Enzymes Metabolic proteins Plasma proteins

AlphaFold DB: P15531

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P15531

HPA link: https://www.proteinatlas.org/ENSG00000239672-NME1

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Cytoplasmic expression in most tissues.

Single cell type specificity Low cell type specificity

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm, Plasma membrane, Cytosol;

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000239672-NME1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000239672-NME1/pathology/endometrial+cancer

OMIM: 156490

OMIM link2: https://www.omim.org/entry/156490

HGNC ID: HGNC:7849

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7849