Biomarker: AIB1

Histology type: endometrial carcinoma

Country: USA

Region: CO

Followed up time :

Subgroup 1 name : Positive in EC

Subgroup 1 number: 79

Subgroup 2 name: negative in EC

Subgroup 2 number: 3

Conclusion: In conclusion, high AIB1 expression in endometrial carcinoma is associated with parameters of poor prognosis. We propose that when AIB1 is overexpressed in endometrial carcinoma, ER action is augmented, leading to endometrial hyperplasia and progression to malignancy. Future studies correlating expression with response to hormonal therapy may be beneficial.

Sample type : tissue

Sample method: immunohistochemistry and tissue microarrays/AIB1 Immunoblot Analysis

Expression pattern : overexpresse

Expression elevation: Staining scores (range 0–300) were calculated for each marker by multiplying the intensity score (0–3) by the percent of cells staining (0–100).

Funtion Uniprot: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

UniProt ID: Q9Y6Q9

UniProt Link: https://www.uniprot.org/uniprotkb/Q9Y6Q9/entry

Biological function from UniProt: #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

Subcellular UniProt: #Cytoplasm #Nucleus

Alternative name from UniProt:

Miscellaneous: NCOA3 is frequently amplified or overexpressed in breast and ovarian cancers.

Catalytic activity: acetyl-CoA + L-lysyl-[protein] = CoA + H+ + N6-acetyl-L-lysyl-[protein]

Activity regulation: Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors. Binds to CSNK1D.

Recommended name: Nuclear receptor coactivator 3

Gene name from HGNC: NCOA3 (ACTR, AIB1, bHLHe42, CAGH16, KAT13B, p/CIP, RAC3, SRC-3, SRC3, TNRC16, TRAM-1)

HPA class: Cancer-related genes Enzymes Metabolic proteins Plasma proteins Transcription factors

AlphaFold DB: Q9Y6Q9

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q9Y6Q9

HPA link: https://www.proteinatlas.org/ENSG00000124151-NCOA3

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic and nuclear expression in all tissues.

Single cell type specificity B-cells - Immune response (mainly)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm, Cytosol

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000124151-NCOA3/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000124151-NCOA3/pathology/endometrial+cancer

Expression figure legend: AIB1 expression

Expression figure link: https://www.nature.com/articles/3800696/figures/3

OMIM: 601937

OMIM link2: https://www.omim.org/entry/601937

HGNC ID: HGNC:7670

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7670