Biomarker: EphB4

Histology type: endometrial carcinoma

Country: Japan

Region: Gifu City

Followed up time :

Conclusion: EphrinB2 and EphB4 were overexpressed during the tumor advancement as dedifferentiation and myometrial invasion. Therefore, ephrinB2/EphB4 might work on tumor advancement and may be recognized as a novel prognostic indicator for uterine endometrial cancers.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : Overexpression

Expression elevation: Intensities were classified as 0 (no staining), 1 (weak staining), 2 (distinct staining), 3 (strong staining) and 4 (very strong staining).

Funtion Uniprot: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

UniProt ID: P54760

UniProt Link: https://www.uniprot.org/uniprotkb/P54760/entry

Biological function from UniProt: #Angiogenesis

Molecular function from UniProt:

Tissue specificity from UniProt: Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Subcellular UniProt: #Cell membrane #Membrane

Alternative name from UniProt:

Catalytic activity: ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]

Recommended name: Ephrin type-B receptor 4

Gene name from HGNC: EPHB4 (HTK, Tyro11)

HPA class: Cancer-related genes Disease related genes Enzymes Human disease related genes Metabolic proteins Plasma proteins Potential drug targets Transporters

AlphaFold DB: P54760

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P54760

Developmental stage: Expressed in fetal heart, lung, liver and to a lower extent in brain. Not expressed in adult brain.

HPA link: https://www.proteinatlas.org/ENSG00000196411-EPHB4

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Cytoplasmic expression in several tissues.

Single cell type specificity Cell type enhanced (Syncytiotrophoblasts, Hepatic stellate cells, Cytotrophoblasts)

Immune cell specificity: Immune cell enhanced (neutrophil, classical monocyte)

Cancer prognostic summary HPA Prognostic marker in colorectal cancer (unfavorable), breast cancer (unfavorable) and urothelial cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000196411-EPHB4/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000196411-EPHB4/pathology/endometrial+cancer

Phenotype ID: 617300

Disease: Lymphatic malformation 7 (LMPHM7)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 600011

OMIM link1: https://www.omim.org/entry/617300

OMIM link2: https://www.omim.org/entry/600011

HGNC ID: HGNC:3395

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3395