Biomarker: NDRG1

Histology type: endometrioid endometrial carcinoma

Stage: early detection

Country: China

Region: Shanghai

Followed up time :

Subgroup 1 name : up-regulated in normal endometrium

Subgroup 1 number: 5

Subgroup 2 name: down-regulated in normal endometrium

Subgroup 2 number: 35

Conclusion: Our results indicated that development of endometrial carcinoma is associated with an overexpression of NDRG1 and the loss of PTEN expression. Identification of changes in the NDRG1 and PTEN expression may be a significant diagnostic tool for the early detection of endometrial carcinoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : overexpression of NDRG1

Expression elevation: In case of a positive signal, NDRG1 protein was located both in the cytoplasm and the membrane of the cells. A semiquantitative method(19) was used: total score was A × B; A denoted the number of positive cells: ≤5% as 0, 6–25% as 1, 26–50% as 2, 51–75% as 3, and >75% as 4; B indicated the positive intensity: colorless as 0, light yellow as 1, yellow as 2, brown as 3. Score 0 as negative (–), 1–4 as weak positive (+), 5–8 as positive (++), and 9–12 as strong positive (+++). If the reading difference between the two pathologists was greater than 3, scoring was

Funtion Uniprot: Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

UniProt ID: Q92597

UniProt Link: https://www.uniprot.org/uniprotkb/Q92597/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

Subcellular UniProt: #Cell membrane #Cytoplasm #Cytoskeleton #Membrane #Microtubule #Nucleus

Alternative name from UniProt:

Recommended name: Protein NDRG1

Gene name from HGNC: NDRG1 (CAP43, DRG1, NDR1, RTP, TDD5)

HPA class: Cancer-related genes Disease related genes Human disease related genes Plasma proteins

AlphaFold DB: Q92597

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q92597

Induction: By homocysteine, 2-mercaptoethanol, tunicamycin in endothelial cells. Induced approximately 20-fold during in vitro differentiation of the colon carcinoma cell lines HT-29-D4 and Caco-2. Induced by oxidative stress in colon cancers. Decreased expression in colon adenomas and adenocarcinomas. Induced by nickel compounds in all tested cell lines. The primary signal for its induction is an elevation of free intracellular calcium ion caused by nickel ion exposure. Okadaic acid, a serine/threonine phosphatase inhibitor, induced its expression more rapidly and more efficiently than nickel.

HPA link: https://www.proteinatlas.org/ENSG00000104419-NDRG1

Tissue specificity RNA from HPA: Tissue enhanced (brain)

Tissue expression from HPA: General cytoplasmic and nuclear expression.

Single cell type specificity Cell type enhanced (Suprabasal keratinocytes, Horizontal cells, Basal keratinocytes, Distal enterocytes)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Microtubules, Cytosol

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable) and renal cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000104419-NDRG1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000104419-NDRG1/pathology/endometrial+cancer

Phenotype ID: 601455

Disease: Charcot-Marie-Tooth disease 4D (CMT4D)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 605262

OMIM link1: https://www.omim.org/entry/601455

OMIM link2: https://www.omim.org/entry/605262

HGNC ID: HGNC:7679

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7679