Biomarker: TFF3

Histology type: endometrioid endometrial carcinoma

Stage: Grade 3

Country: Italy

Region: Brescia

Followed up time :

Subgroup 1 name : low expression(0-1) in EEC

Subgroup 1 number: 23

Subgroup 2 name: high expression(2-3) in EEC

Subgroup 2 number: 15

Conclusion: In conclusion, TFF3 is highly expressed at gene and protein level in G3-EEC. Further investigations on a wider set of samples are warranted to validate TFF3 as a novel serum marker for early detection and/or monitoring of G3-EEC patients.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression（2-3）

Expression elevation: The staining and on the percentage of tumour cell stained. Intensity was scored as follows: 0 indicating no staining; 1 weak staining; 2 moderate staining; and 3 strong staining. The percentage of tumour cells stained was scored as follows: 0 indicating no staining; 1 indicating 1–10%, 2 indicating 11–50%, and 3 indicating 51–100%. Then, multiplying the intensity score against the percentage staining score, we obtained a single scale with scores of 0, 1, 2, 3, 4, 6, and 9. A total score was calculated grouping scores 1–3 in total score 1, 4, and 6 in total score 2 and 9 in total score 3. Tissues with no staining in term of intensity and percentage of positive cells (total score=0) were scored as negative.

Funtion Uniprot: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

UniProt ID: Q07654

UniProt Link: https://www.uniprot.org/uniprotkb/Q07654/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level).

Subcellular UniProt: #Cytoplasm #Extracellular matrix #Secreted

Alternative name from UniProt:

Recommended name: Trefoil factor 3

Gene name from HGNC: TFF3 (HITF, ITF)

HPA class: Cancer-related genes Plasma proteins

AlphaFold DB: Q07654

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q07654

HPA link: https://www.proteinatlas.org/ENSG00000160180-TFF3

Tissue specificity RNA from HPA: Group enriched (cervix, intestine, salivary gland, thyroid gland)

Tissue expression from HPA: Distinct expression in mucus secreting cells in gastrointestinal tract, cervix uteri and respiratory epithelia, with secreted positivity in thyroid gland.

Single cell type specificity Cell type enriched (Intestinal goblet cells)

Immune cell specificity: Immune cell enriched (eosinophil)

Subcellular summary HPA Located in Nucleoplasm, Nucleoli (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in endometrial cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000160180-TFF3/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000160180-TFF3/pathology/endometrial+cancer

Expression figure legend: Representative immunohistochemical staining for TFF3

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528153/figure/fig2/

OMIM: 600633

OMIM link2: https://www.omim.org/entry/600633

HGNC ID: HGNC:11757

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:11757