Biomarker: HAI-2

Histology type: endometrial carcinoma

Country: Japan

Region: Okayama

Followed up time :

Subgroup 1 name : weak epithelial staining

Subgroup 1 number: 21

Subgroup 2 name: moderate staining;high staining

Subgroup 2 number: 25;40

Conclusion: These findings indicate that HAI-2 could be considered as therapeutic targets for endometrial cancer.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : (score 2)

Expression elevation: The level of HAI-2 staining in epithelial cells was classified into three groups by scoring the percentage of positive cells: strong (2); >50% of cells stained, moderate (1); 10–50% of cells stained, and weak (0); <10% of cells stained. Low epithelial expression, score 0–1; high epithelial expression, score 2

Statictics: cutoff<60;cutoff>60

Cohort age: 32;24

Funtion description: HAI-1 and HAI-2 showed potential inhibitory effects on cell proliferation, migration and cellular invasion by reduction of matriptase and hepsin expression. This in turn led to an increase in the levels of E-cadherin and Slug, and a reduction in the levels of Vimentin, SIP1, Snail and Twist, and hence ER and PR signal transduction in endometrial cancer cells.

Funtion Uniprot: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa.

UniProt ID: O43291

UniProt Link: https://www.uniprot.org/uniprotkb/O43291/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea.

Subcellular UniProt: #Membrane

Alternative name from UniProt:

Recommended name: Kunitz-type protease inhibitor 2

Gene name from HGNC: SPINT2 (HAI-2, Kop)

HPA class: Cancer-related genes Disease related genes Human disease related genes Plasma proteins

AlphaFold DB: O43291

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O43291

HPA link: https://www.proteinatlas.org/ENSG00000167642-SPINT2

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic expression.

Single cell type specificity Cell type enhanced (Syncytiotrophoblasts, Cytotrophoblasts)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Plasma membrane, Intermediate filaments, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000167642-SPINT2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000167642-SPINT2/pathology/endometrial+cancer

Phenotype ID: 270420

Disease: Diarrhea 3, secretory sodium, congenital, with or without other congenital anomalies (DIAR3)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 605124

OMIM link1: https://www.omim.org/entry/270420

OMIM link2: https://www.omim.org/entry/605124

HGNC ID: HGNC:11247

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:11247