Biomarker: HE4

Histology type: endometrial carcinoma

Stage: high risk

Country: Italy

Region: Brescia

Followed up time :

Conclusion: Protein and HE4 gene were significantly upregulated in EC tissues and sera, compared with controls. High sHE4 levels were significantly associated with worse EC clinical characteristics

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: Cytoplasmic staining was graded for intensity (0-negative, 1-weak, 2-moderate and 3-strong) and the percentage of positive cells was scored as 0 (0%), 1 (1–10%), 2 (11–50%) and 3 (51–100%). A single scale with scores 0–9 was obtained by multiplying the intensity and the percentage staining score, and a total score was calculated by grouping score 0 in total score 0, 1–3 in total score 1, 4 and 6 in total score 2 and 9 in total score 3.

Statictics: cutoff<65;cutoff>65

Cohort age: 60;78

Funtion Uniprot: Broad range protease inhibitor.

UniProt ID: Q14508

UniProt Link: https://www.uniprot.org/uniprotkb/Q14508/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

Subcellular UniProt: #Secreted

Alternative name from UniProt:

Recommended name: WAP four-disulfide core domain protein 2

Gene name from HGNC: WFDC2 (dJ461P17.6, EDDM4, HE4, WAP5)

HPA class: Cancer-related genes

AlphaFold DB: Q14508

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q14508

HPA link: https://www.proteinatlas.org/ENSG00000101443-WFDC2

Tissue specificity RNA from HPA: Tissue enhanced (cervix, salivary gland)

Tissue expression from HPA: Cytoplasmic expression in selected tissues.

Single cell type specificity Cell type enhanced (Club cells, Glandular and luminal cells, Basal respiratory cells, Urothelial cells, Endometrial ciliated cells, Respiratory ciliated cells, Collecting duct cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Secreted

Cancer prognostic summary HPA Prognostic marker in glioma (unfavorable) and renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000101443-WFDC2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000101443-WFDC2//pathology/endometrial+cancer

Expression figure legend: Representative immunohistochemical staining for HE4

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101927/figure/fig2/

Survival figure legend: Kaplan–Meier survival curves for EC patients according to sHE4 levels on the entire patient cohort

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3101927/figure/fig4/

OMIM: 617548

OMIM link2: https://www.omim.org/entry/617548

HGNC ID: HGNC:15939

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:15939