Biomarker: MMP-9 

Histology type: endometrial carcinoma

Country: Germany

Region:  Kiel

Followed up time :

Conclusion: There are obvious differences in expression patterns of MMP-9 in different stages of endometriosis and in endometrial cancer. 

Sample type : tissue

Sample method: immunohistochemisty

Expression pattern : expression

Expression elevation: Staining intensity (SI) was multiplied by the percentage of positive cells (PP) gaining a score between 0 and 12. PP considered 5 groups: >80% of positive (four points), 51–80% positive cells (three points), two points for 10–50%, one point for <10% and zero points if no positive cells could be detected. Staining intensity ranged from no reaction = 0 points, slightly stained = 1 point, moderately stained = 2 points and strongly stained = 3 points. A score >3 was evaluated as a positive reaction and 9 points as strongly positive.

Funtion Uniprot: Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, PubMed:1480034, PubMed:12879005). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide

UniProt ID: P14780

UniProt Link: https://www.uniprot.org/uniprotkb/P14780/entry

Biological function from UniProt: #Collagen degradation

Molecular function from UniProt:

Tissue specificity from UniProt: Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes

Subcellular UniProt: #Extracellular matrix #Secreted

Alternative name from UniProt:

Cleaved: Cleaved into 2 chains 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9

Miscellaneous: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.

Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V.

Activity regulation: Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1.

Recommended name: Matrix metalloproteinase-9

Gene name from HGNC: MMP9 (CLG4B)

HPA class: Cancer-related genes Candidate cardiovascular disease genes Disease related genes Enzymes FDA approved drug targets Human disease related genes Plasma proteins

AlphaFold DB: P14780

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P14780

Induction: Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. (Microbial infection) Expression induced by M.bovis MPB83 (at protein level) (PubMed:20800577).

HPA link: https://www.proteinatlas.org/ENSG00000100985-MMP9

Tissue specificity RNA from HPA: Group enriched (bone marrow, lymphoid tissue)

Tissue expression from HPA: Selective nuclear and cytoplasmic expression in a subset of immune cells in several tissues, including lymphoid organs, and hematopoietic cells.

Single cell type specificity Group enriched (Langerhans cells, Hofbauer cells, Macrophages)

Immune cell specificity: Immune cell enriched (neutrophil)

Subcellular summary HPA Located in Cytosol

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable), endometrial cancer (favorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000100985-MMP9/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000100985-MMP9/pathology/endometrial+cancer

Phenotype ID: 03932;613073;

Disease: Intervertebral disc disease (IDD);Metaphyseal anadysplasia 2 (MANDP2);

Note1: Disease susceptibility is associated with variants affecting the gene represented in this entry;The disease is caused by variants affecting the gene represented in this entry;

Note2: A number sign (#) is used with this entry because of evidence that variations in many genes are involved in susceptibility to intervertebral disc disease. See MOLECULAR GENETICS section.;A number sign (#) is used with this entry because of evidence that metaphyseal anadysplasia-2 (MANDP2) is caused by homozygous mutation in the gene encoding matrix metalloproteinase-9 (MMP9; 120361) on chromosome 20q13. For a general phenotypic description and a discussion of genetic heterogeneity of metaphyseal anadysplasia, see MANDP1 (602111).;

OMIM: 120361;603932;613073;

OMIM link1: https://www.omim.org/entry/603932;https://www.omim.org/entry/613073;

OMIM link2: https://www.omim.org/entry/120361;https://www.omim.org/entry/613073;

Phenotype: INTERVERTEBRAL DISC DISEASE;METAPHYSEAL ANADYSPLASIA 2;

HGNC ID: HGNC:7176

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7176