Biomarker: MMP-2

Histology type: endometrial carcinoma

Country: Germany

Region:  Kiel

Followed up time :

Conclusion: There are obvious differences in expression patterns of MMP-2, in different stages of endometriosis and in EC.

Sample type : tissue

Sample method: immunohistochemisty

Expression pattern : expression

Expression elevation: Staining intensity (SI) was multiplied by the percentage of positive cells (PP) gaining a score between 0 and 12. PP considered 5 groups: >80% of positive (four points), 51–80% positive cells (three points), two points for 10–50%, one point for <10% and zero points if no positive cells could be detected. Staining intensity ranged from no reaction = 0 points, slightly stained = 1 point, moderately stained = 2 points and strongly stained = 3 points. A score >3 was evaluated as a positive reaction and 9 points as strongly positive.

Funtion Uniprot: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2 Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

UniProt ID: P08253

UniProt Link: https://www.uniprot.org/uniprotkb/P08253/entry

Biological function from UniProt: #Angiogenesis #Collagen degradation

Molecular function from UniProt:

Tissue specificity from UniProt: Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.

Subcellular UniProt: #Cytoplasm #Extracellular matrix #Membrane #Mitochondrion #Nucleus #Secreted

Alternative name from UniProt:

Cleaved: Cleaved into 1 chains PEX

Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Activity regulation: Inhibited by histatin-3 1/24 (histatin-5).

Recommended name: 72 kDa type IV collagenase

Gene name from HGNC: MMP2 (CLG4, CLG4A, TBE-1)

HPA class: Cancer-related genes Candidate cardiovascular disease genes Disease related genes Enzymes Human disease related genes Plasma proteins Potential drug targets

AlphaFold DB: P08253

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P08253

Induction: Aspirin appears to inhibit expression.

HPA link: https://www.proteinatlas.org/ENSG00000087245-MMP2

Tissue specificity RNA from HPA: Tissue enhanced (gallbladder)

Tissue expression from HPA: Cytoplasmic expression in respiratory epithelia and placenta as well as endothelial and stromal cells in most tissues.

Single cell type specificity Fibroblasts - ECM organization (mainly)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Vesicles

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000087245-MMP2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000087245-MMP2/pathology/endometrial+cancer

Phenotype ID: 259600

Disease: Multicentric osteolysis, nodulosis, and arthropathy (MONA)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 120360

OMIM link1: https://www.omim.org/entry/259600

OMIM link2: https://www.omim.org/entry/120360

HGNC ID: HGNC:7166

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7166