Biomarker: podocalyxin

Histology type: endometrioid endometrial carcinoma

Country: Japan

Region: Wakayama City

Followed up time :

Subgroup 1 name : Negative

Subgroup 1 number: 39

Subgroup 2 name: Positive

Subgroup 2 number: 22

Conclusion: These results suggest that overexpression of podocalyxin and pEzrin may indicate a more aggressive phenotype; thus, evaluation of these proteins may be useful in prediction of disease progression in UEA cases.

Sample type : tissue

Sample method: immunohistochemisty

Expression pattern : expresssion

Expression elevation: Samples were considered positive for podocalyxin if cytoplasmic immunoreactivity was detected in at least 10% of the analysed cells, according to the mean immunopositive area 6SD (mean podocalyxin positive area 9.7%62.5%)

Statictics: cutoff<59;cutoff>59

Cohort age: 34;27

Subgroup 1 age: 23;16

Subgroup 2 age: 11;11

Funtion Uniprot: Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

UniProt ID: O00592

UniProt Link: https://www.uniprot.org/uniprotkb/O00592/entry

Biological function from UniProt: #Cell adhesion

Molecular function from UniProt:

Tissue specificity from UniProt: Glomerular epithelium cell (podocyte).

Subcellular UniProt: #Cell membrane #Cell projection #Membrane

Alternative name from UniProt:

Recommended name: Podocalyxin

Gene name from HGNC: PODXL (Gp200, PC, PCLP)

HPA class: Plasma proteins

AlphaFold DB: O00592

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O00592

HPA link: https://www.proteinatlas.org/ENSG00000128567-PODXL

Tissue specificity RNA from HPA: Tissue enhanced (kidney)

Tissue expression from HPA: Distinct expression in glomerular podocytes, endothelial cells, glandular cells in fallopian tube, uterus and seminal vesicle.

Single cell type specificity Cell type enhanced (Rod photoreceptor cells, Endothelial cells, Adipocytes, Glandular and luminal cells, Microglial cells, Cone photoreceptor cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Vesicles, Plasma membrane, Centriolar satellite

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000128567-PODXL/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000128567-PODXL/pathology/endometrial+cancer

OMIM: 602632

OMIM link2: https://www.omim.org/entry/602632

HGNC ID: HGNC:9171

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:9171