Biomarker: bFGF

Histology type: endometrial carcinoma

Country: USA

Region: Pittsburgh,PA

Followed up time :

Subgroup 1 name : Tumour bFGF expression

Subgroup 1 number: 35

Subgroup 2 name: Stromal bFGF expression

Subgroup 2 number: 116

Conclusion: These IHC data show that tumour and stromal bFGF expression have opposite associations with survival outcomes in EC patients. If confirmed in larger studies, tumour-derived bFGF could be an attractive target in EC therapy.

Sample type : tissue

Sample method: Immunohistochemistry

Expression pattern : expression (negative (0) vs positive (2–7))

Expression elevation: The intensity of staining (0=none, 1=weak, 2=moderate, and 3=strong) and the proportion of positive-staining cells (1=1–5%, 2=6–20%, 3=21–80%, and 4=>80%) were summed to calculate the cumulative score. Acceptable values of the cumulative score are 0, 2, 3, 4, 5, 6, and 7. As the prevalence of positive expression scores (cumulative scores 2–7) was low for HGF and c-Met, we chose to categorise expression as negative (0) vs positive (2–7). bFGF expression was apparent in the tumour and stroma.

Statictics: cutoff<65;cutoff>65

Cohort age: 82;69

Subgroup 1 age: 17;18

Subgroup 2 age: 65;51

Funtion Uniprot: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879).

UniProt ID: P09038

UniProt Link: https://www.uniprot.org/uniprotkb/P09038/entry

Biological function from UniProt: #Angiogenesis #Differentiation

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Subcellular UniProt: #Nucleus #Secreted

Alternative name from UniProt:

Miscellaneous: This protein binds heparin more strongly than does aFGF.;pH Dependence Retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degrees Celsius.1 Publication Temperature Dependence Inactivated after 3 minutes at 60 degrees Celsius or 1 minute at 80 degrees Celsius;

Recommended name: Fibroblast growth factor 2

Gene name from HGNC: FGF2 (FGFB)

HPA class: Cancer-related genes Candidate cardiovascular disease genes FDA approved drug targets RAS pathway related proteins Transporters

AlphaFold DB: P09038

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P09038

HPA link: https://www.proteinatlas.org/ENSG00000138685-FGF2

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Nuclear expression in most tissues, secreted positivity enriched in brain.

Single cell type specificity Cell type enhanced (Astrocytes, Fibroblasts, Oligodendrocyte precursor cells, Endometrial stromal cells, Adipocytes)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Nucleoplasm, Nuclear bodies

Cancer prognostic summary HPA Prognostic marker in pancreatic cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000138685-FGF2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000138685-FGF2/pathology/endometrial+cancer

Expression figure legend: Representative IHC stains showing (A) negative HGF expression, (B and C) positive HGF expression

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388566/figure/fig1/

Survival figure legend: Kaplan–Meier curves of (A) OS and (B) RFS by tumour bFGF status.

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388566/figure/fig2/

OMIM: 134920

OMIM link2: https://www.omim.org/entry/134920

HGNC ID: HGNC:3676

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3676