Biomarker: E-cadherin

Histology type: endometrial carcinoma

Country: Japanese

Region: Osaka

Followed up time :

Subgroup 1 name : preserved

Subgroup 1 number: 213

Subgroup 2 name: reduced

Subgroup 2 number: 141

Conclusion: The current study is the first report to demonstrate that the EMT status, as represented by both reduced E-cadherin expression and nuclear Snail expression, was identified to be an independent predictive factor of patient survival in endometrial cancer.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : EMT (reduced E-cadherin and nuclear expression of Snail )

Expression elevation: E-cadherin expression was scored as: 0 (no stain), 1+ (low intensity immunoreactivity in more than 10% of tumor cells), 2+ (medium intensity immunoreactivity of more than 10% of tumor cells), 3+ (high intensity immunoreactivity of more than 10% of tumor cells). These data were summarized into two groups; preserved E-cadherin (3+) and reduced E-cadherin (0, 1+, 2+). The Snail and Slug expressions were evaluated as positive only when nuclear staining was detectable: 0 (no stain), 1+ (immunoreactivity of more than 1% of tumor cells), 2+ (immunoreactivity of more than 2–5% of tumor cells), 3+ (immunoreactivity of more than 5% of tumor cells), then divided into two groups, negative (0), and positive (1+, 2+, 3+). Scoring was performed three times per slide for three distinct fields, and the three scores were averaged.

Statictics: cutoff<50;cutoff>50

Cohort age: 68;186

Subgroup 1 age: 57;156

Subgroup 2 age: 11;130

Funtion Uniprot: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-72 Publications E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.1 Publication (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.3 Publications

UniProt ID: P12830

UniProt Link: https://www.uniprot.org/uniprotkb/P12830/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Non-neural epithelial tissues.

Subcellular UniProt: #Cell junction #Cell membrane #Endosome #Golgi apparatus #Membrane

Alternative name from UniProt:

Recommended name: Cadherin-1

Gene name from HGNC: CDH1 (CD324, UVO, uvomorulin)

HPA class: Cancer-related genes CD markers Disease related genes Human disease related genes Plasma proteins

AlphaFold DB: P12830

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P12830

Induction: Expression is repressed by MACROD1.

HPA link: https://www.proteinatlas.org/ENSG00000039068-CDH1

Tissue specificity RNA from HPA: Tissue enhanced (parathyroid gland)

Tissue expression from HPA: Cytoplasmic and membranous expression in epithelial cells.

Single cell type specificity Cell type enhanced (Distal enterocytes, Glandular and luminal cells, Pancreatic endocrine cells)

Immune cell specificity: Group enriched (plasmacytoid DC, myeloid DC)

Subcellular summary HPA Located in Golgi apparatus, Plasma membrane, Cell Junctions (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000039068-CDH1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000039068-CDH1/pathology/endometrial+cancer

Expression figure legend: Survival curves generated by using the Kaplan-Meier method in 354 endometrial cancer patients.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566047/figure/F2/?report=objectonly

Phenotype ID: 137215;608089

Disease: Hereditary diffuse gastric cancer (HDGC);Endometrial cancer (ENDMC)| Ovarian cancer (OC)|Breast cancer, lobular (LBC)|Blepharocheilodontic syndrome 1 (BCDS1)

Note1: Disease susceptibility is associated with variants affecting the gene represented in this entry. Heterozygous CDH1 germline mutations are responsible for familial cases of diffuse gastric cancer. Somatic mutations has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer;Disease susceptibility is associated with variants affecting the gene represented in this entry| Disease susceptibility is associated with variants affecting the gene represented in this entry |The gene represented in this entry may be involved in disease pathogenesis |The disease is caused by variants affecting the gene represented in this entry|

OMIM: 192090

OMIM link1: https://www.omim.org/entry/137215|https://www.omim.org/entry/608089|https://www.omim.org/entry/137215|https://www.omim.org/entry/119580

OMIM link2: https://www.omim.org/entry/192090

HGNC ID: HGNC:1748

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1748