Biomarker: S38

Histology type: endometrial carcinoma

Stage: primary

Country: Norway

Region: Bergen

Study type: retrospective study

Followed up time :

Subgroup 1 name : Primary investigation series Low expression

Subgroup 1 number: 370

Subgroup 2 name: Primary investigation series high expression

Subgroup 2 number: 149

Conclusion: High pStathmin(S38) is a novel biomarker of increased tumor cell proliferation and impaired prognosis as reported here for independent cohorts of endometrial cancer and not previously shown in human cancer.

Sample type : tissue

Sample method: immunohistochemistry, FISH, flow cytometry, DNA oligonucleotide microarray, single-nucleotide polymorphism array, and Sanger sequencing

Expression pattern : high expression(SI > 4)

Expression elevation: A semiquantitative grading system incorporating staining intensity (score, 0–3) and area of tumor with positive staining (0, no staining; 1, <10%; 2, 10%–50%; and 3, >50% of tumor cells) was applied. The staining index (SI) was calculated as the product of staining intensity and area ranging from 0 to 9, as described in several publications. If heterogeneity was seen for the 3 cylinders of each case, the 3 cylinders were given one overall averaged score in a manner similar to the approach applied for investigations of full sections for comparison (For cutoff defining methods, see "Statistical analyses"). A cutoff representing the upper quartile (SI > 4) was used to define high level of pStathmin(S38). For Stathmin, the upper quartile (SI= 9) defined high immunohistochemical expression, as previously reported.

Statictics: cutoff<65;cutoff>65

Cohort age: 252;266

Subgroup 1 age: 187;183

Subgroup 2 age: 65;84

UniProt ID: A1L453

UniProt Link: https://www.uniprot.org/uniprotkb/A1L453/entry

Molecular function from UniProt:

Subcellular UniProt: #Secreted

Alternative name from UniProt:

Recommended name: Serine protease 38

Gene name from HGNC: PRSS38 (MPN2)

HPA class: Enzymes

AlphaFold DB: A1L453

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/A1L453

HPA link: https://www.proteinatlas.org/ENSG00000185888-PRSS38

Tissue specificity RNA from HPA: Tissue enriched (testis)

Single cell type specificity Group enriched (Spermatocytes, Spermatogonia)

Immune cell specificity: Not detected in immune cells

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000185888-PRSS38/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000185888-PRSS38/pathology/endometrial+cancer

HGNC ID: HGNC:29625

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:29625