Biomarker: C2GnT1

Histology type: endometrial carcinoma

Country: Japan

Region: Matsumoto

Followed up time :

Conclusion: C2GnT1 appears to be involved in the biological aggressiveness of endometrial carcinoma. C2GnT1 might become a novel prognostic factor for endometrial carcinoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : overexpression( positivity index ≥ 10)

Expression elevation: Immunoreactivity was evaluated according to the percentage of positive cells, which was defined as staining similar to or stronger than that in plasma cells, among 500 cells in five randomly selected high-power fields, and these results were described as a positivity index (PI) with a maximal score of 100. Cases with PI < 1 were defined as negative (-),cases with 1 ≤ PI < 5 as focal positive (±_x005f_x0001_), cases with5 ≤ PI < 10 as positive (+), and cases with PI≥ 10 as extensive positive (2+).

Funtion Uniprot: Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan (PubMed:1329093, PubMed:23027862). The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor (By similarity). Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites (By similarity). Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant (By similarity). Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates (By similarity).By Similarity2 Publications

UniProt ID: Q02742

UniProt Link: https://www.uniprot.org/uniprotkb/Q02742/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Highly expressed in activated T-lymphocytes and myeloid cells.

Subcellular UniProt: #Golgi apparatus #Membrane

Alternative name from UniProt:

Catalytic activity: O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + H+ + UDP1 Publication This reaction proceeds in the forward direction.1 Publication EC:2.4.1.102 (UniProtKB | ENZYME | Rhea) | O3-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + H+ + UDP1 Publication This reaction proceeds in the forward direction.1 Publication EC:2.4.1.102 (UniProtKB | ENZYME | Rhea)| globoside GalGb4Cer + UDP-N-acetyl-alpha-D-glucosamine = globoside GlcNAc-(beta1->6)-GalGb4Cer + H+ + UDPBy Similarity This reaction proceeds in the forward direction.By Similarity Source: Rhea 56900| ganglioside GA1 + UDP-N-acetyl-alpha-D-glucosamine = ganglioside beta-DGlcNAc-(1->6)-GA1 + H+ + UDPBy Similarity This reaction proceeds in the forward direction.By Similarity Source: Rhea 69691|

Recommended name: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene name from HGNC: GCNT1 (C2GNT, NACGT2, NAGCT2)

HPA class: Enzymes Metabolic proteins

AlphaFold DB: Q02742

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q02742

HPA link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:4203

Tissue specificity RNA from HPA: Group enriched (intestine, parathyroid gland, stomach, thyroid gland)

Tissue expression from HPA: Cytoplasmic expression in most tissues.

Single cell type specificity Cell type enhanced (Gastric mucus-secreting cells, Kupffer cells, Proximal enterocytes)

Immune cell specificity: Immune cell enhanced (eosinophil)

Subcellular summary HPA Located in Nuclear speckles

Cancer prognostic summary HPA Prognostic marker in urothelial cancer (unfavorable) and endometrial cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000187210-GCNT1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000187210-GCNT1/pathology/endometrial+cancer

OMIM: 600391

OMIM link2: https://www.omim.org/entry/600391

HGNC ID: HGNC:4203

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:4203