Biomarker: CYP1A1

Histology type: endometrial carcinoma

Country: Greece

Region: Athens

Followed up time :

Subgroup 1 name : overexpressed CYP1A1 mRNA

Subgroup 1 number: 5

Subgroup 2 name: downregulated CYP1A1 mRNA

Subgroup 2 number: 9

Conclusion: Overexpression of active CYP1 enzymes in 21 % of the tumors highlights the potential use of the latter enzymes as chemotherapeutic targets in endometrial cancer.

Sample type : tissue

Sample method: qRT-PCR/Western blotting,enzyme activity assays,HPLC and mass spectrometry analysis

Expression pattern : overexpression

Statictics: Mean ;Range

Cohort age: 63.7;46–72

Funtion Uniprot: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).

UniProt ID: P04798

UniProt Link: https://www.uniprot.org/uniprotkb/P04798/entry

Biological function from UniProt: #Fatty acid metabolism #Lipid biosynthesis #Lipid metabolism #Steroid biosynthesis

Molecular function from UniProt:

Tissue specificity from UniProt: Lung, lymphocytes and placenta.

Subcellular UniProt: #Cytoplasm #Endoplasmic reticulum #Membrane #Microsome #Mitochondrion #Mitochondrion inner membrane

Alternative name from UniProt:

Catalytic activity: https://www.uniprot.org/uniprotkb/P04798/entry#function

Recommended name: Cytochrome P450 1A1

Gene name from HGNC: CYP1A1 (CP11, CYP1, P1-450, P450-C, P450DX)

HPA class: Enzymes Metabolic proteins Plasma proteins

AlphaFold DB: P04798

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P04798

Induction: By 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

HPA link: https://www.proteinatlas.org/ENSG00000140465-CYP1A1

Tissue specificity RNA from HPA: Tissue enhanced (liver, urinary bladder)

Tissue expression from HPA: General cytoplasmic expression.

Single cell type specificity Cell type enhanced (Adipocytes, Urothelial cells, Endothelial cells, Hepatocytes, Glandular and luminal cells)

Immune cell specificity: Not detected in immune cells

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000140465-CYP1A1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000140465-CYP1A1/pathology/endometrial+cancer

OMIM: 108330

OMIM link2: https://www.omim.org/entry/108330

HGNC ID: HGNC:2595

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:2595