Biomarker: HER-2/neu

Histology type: endometrioid endometrial carcinoma

Country: Greece

Region: Thessaloniki

Followed up time :

Conclusion: HER-2/neu is an independent prognostic factor for type I endometrial carcinoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: HER-2/neu expression was scored according to the FDA approved DAKO Hercept Test protocol system guidelines (scores from 0 to 3+). Specifically, cases were considered as negative if showed no staining or cell membrane staining in less than 10 % of the tumor cells (Score 0) or faint perceptible membrane staining in more than 10 % of the tumor cells, respectively (Score 1+). A weak to moderate complete membrane staining observed in more than 10 % of the tumor cells interpreted as ‘‘weakly positive’’ (Score 2+), whereas in Score 3+, a strong complete membrane staining was observed in more than 10 % of the tumor cells. Cases with HER-2/neu Score of 2+ and 3+ in more than 10 % of the tumor cells were considered as positive.

Statictics: Mean

Cohort age: 62.7

Funtion Uniprot: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.Curated In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.3 Publications

UniProt ID: P04626

UniProt Link: https://www.uniprot.org/uniprotkb/P04626/entry

Biological function from UniProt: Transcription, Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth

Subcellular UniProt: #Cell membrane #Cytoplasm #Endosome #Membrane #Nucleus

Alternative name from UniProt:

Catalytic activity: ATP + L-tyrosyl-[protein] = ADP + H+ + O-phospho-L-tyrosyl-[protein]

Activity regulation: Activated by dimerization. Not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.

Gene name from HGNC: ERBB2 (CD340, HER-2, HER2, NEU, NGL)

CD antigen name: CD340

HPA link: https://www.proteinatlas.org/ENSG00000141736-ERBB2

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Cytoplasmic and membranous expression in most tissues.

Subcellular summary HPA Located in Plasma membrane, Cytosol

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable), endometrial cancer (unfavorable) and pancreatic cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000163507-CIP2A/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000163507-CIP2A/pathology/endometrial+cancer

OMIM: 137800;164870;167000;

OMIM link2: https://www.omim.org/entry/137800;https://www.omim.org/entry/164870;https://www.omim.org/entry/167000;

Phenotype: GLIOMA SUSCEPTIBILITY 1; GLM1;ERB-B2 RECEPTOR TYROSINE KINASE 2; ERBB2;

HGNC ID: HGNC:3430

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3430