Biomarker: WT-1

Histology type: endometrial serous carcinomas

Followed up time :

Subgroup 1 name : Negative

Subgroup 1 number: 43

Subgroup 2 name: Positive

Subgroup 2 number: 34

Conclusion: Our results show that WT-1 has prognostic value, being predictive of DFS. As a potential prognostic marker and therapeutic target, we recommend that WT-1 expression should be included in histopathologic reports of endometrial serous carcinoma.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: Expression of WT-1 was considered positive when nuclear staining was identified, as this is the profile considered to be positive in routine clinical practice

Statictics: Median;Range

Cohort age: 69;45-88

Funtion Uniprot: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

UniProt ID: P19544

UniProt Link: https://www.uniprot.org/uniprotkb/P19544/entry

Biological function from UniProt: #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in the kidney and a subset of hematopoietic cells.

Subcellular UniProt: #Cytoplasm #Nucleus

Alternative name from UniProt:

Miscellaneous: Presence of the KTS motif hinders interactions between DNA and zinc-finger 4.

Recommended name: Wilms tumor protein

Gene name from HGNC: WT1 (AWT1, GUD, NPHS4, WAGR, WIT-2)

HPA class: Cancer-related genes Disease related genes Human disease related genes Transcription factors

AlphaFold DB: P19544

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P19544

HPA link: https://www.proteinatlas.org/ENSG00000184937-WT1

Tissue specificity RNA from HPA: Tissue enhanced (endometrium, fallopian tube, ovary)

Tissue expression from HPA: Nuclear expression in few tissues including fallopian tube, endometrium and testis.

Single cell type specificity Cell type enhanced (Endometrial stromal cells, Collecting duct cells, Endothelial cells, Granulosa cells, Late spermatids)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Nucleoplasm

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000184937-WT1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000184937-WT1/pathology/endometrial+cancer

Phenotype ID: 136680 $ 194070 $194080 $ 256370 $ 608978 $ $ 156240$

Disease: Frasier syndrome (FS)$ Wilms tumor 1 (WT1)$Denys-Drash syndrome (DDS) $ Nephrotic syndrome 4 (NPHS4)$ Meacham syndrome (MEACHS) $Mesothelioma, malignant (MESOM) $

Note1: The disease is caused by variants affecting the gene represented in this entry $The disease is caused by variants affecting the gene represented in this entry $The disease is caused by variants affecting the gene represented in this entry $ The disease is caused by variants affecting the gene represented in this entry $ The disease is caused by variants affecting the gene represented in this entry $ A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1$ The disease may be caused by variants affecting the gene represented in this entry $

OMIM link1: https://www.omim.org/entry/136680 $https://www.omim.org/entry/194070 $ https://www.omim.org/entry/194080 $ https://www.omim.org/entry/256370 $https://www.omim.org/entry/608978 $ $https://www.omim.org/entry/156240

HGNC ID: HGNC:12796

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:12796