Biomarker: mesothelin

Histology type: endometrial carcinoma

Country: Japan

Region: Tokorozawa

Followed up time :

Subgroup 1 name : positive

Subgroup 1 number: 171

Subgroup 2 name: negative

Subgroup 2 number: 314

Conclusion: Mesothelin expression and co-expression might be associated with tumor aggressiveness and poor prognosis in patients with endometrial carcinoma. Persons with mesothelin-expressing endometrial cancers present a particularly high medical unmet need.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression( The percentage of mesothelin-positive cells is ≥50% (tumor proportion score: 3+), and the entire circumference of the cancer cell membrane is stained (intensity score: 2+))

Expression elevation: The tumor proportion score, defined as the percentage of mesothelin-positive cells in carcinoma tissues, was as follows:0, no tumor cells stained in the entire carcinoma tissue; 1+, ≥1 to < 10% of cells stained in the entire carcinoma tissue; 2+, ≥10 to < 50% of cells stained in the entire carcinoma tissue; and 3+, ≥50% of cells stained in the entire carcinoma tissue. The staining intensity score was defined as follows: 0, no tumor cells stained in the entire carcinoma tissue; 1+, incomplete membrane staining and/or faint or barely perceptible cytoplasmic staining detected; and 2+, entire circumference of the cell membrane stained and/or moderate to strong cytoplasmic staining.

Statictics: cutoff<60;cutoff>60

Cohort age: 233;252

Subgroup 1 age: 72;99

Subgroup 2 age: 161;153

Funtion Uniprot: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

UniProt ID: Q13421

UniProt Link: https://www.uniprot.org/uniprotkb/Q13421/entry#function

Biological function from UniProt: Cell adhesion

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Subcellular UniProt: #Cell membrane #Golgi apparatus #Membrane #Secreted

Alternative name from UniProt:

Recommended name: Mesothelin

Gene name from HGNC: MSLN (CAK1, MPF)

HPA class: Located in Nucleoplasm, Vesicles

AlphaFold DB: Q13421

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q13421

HPA link: https://www.proteinatlas.org/ENSG00000102854-MSLN

Tissue specificity RNA from HPA: Tissue enhanced (adipose tissue, fallopian tube)

Tissue expression from HPA: Cytoplasmic and membranous expression in squamous epithelia and glandular cells of cervix and fallopian tube.

Single cell type specificity Cell type enhanced (Paneth cells, Alveolar cells type 1, Club cells, Alveolar cells type 2, Glandular and luminal cells)

Immune cell specificity: Respiratory epithelial cells - Mucosal defense (mainly)

Subcellular summary HPA Located in Nucleoplasm, Vesicles

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable) and pancreatic cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000102854-MSLN/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000102854-MSLN/pathology/endometrial+cancer

Expression figure legend: The expression variation of mesothelin and cancer antigen (CA) 125

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034188/figure/Fig1/

Survival figure legend: Progression-free survival (PFS) and overall survival (OS) of patients according to their differences in expression of mesothelin and CA125, and in the co-expression of both mesothelin and CA125.

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034188/figure/Fig2/

Disease: No disease ID

Note1: Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer

OMIM: 601051

OMIM link2: https://www.omim.org/entry/601051

HGNC ID: HGNC:7371

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:7371