Biomarker: FolR1

Histology type: endometrial serous carcinomas

Country: Spain

Region: Lleida

Followed up time :

Subgroup 1 name : EEC

Subgroup 1 number: 57

Subgroup 2 name: SC

Subgroup 2 number: 31

Conclusion: We propose an internally and externally validated 9-protein biomarker signature to predict the histologic type of EC (EEC or SC) by IHC. The results also suggest that mixed EEC-SC is molecularly ambiguous.

Sample type : tissue

Sample method: immunohistochemistry (IHC)

Expression pattern : FolR1 ≥ 50

Expression elevation: IHC staining was graded semiquantitatively by considering the percentage and intensity of the staining. A histologic score (Hs) was obtained from each sample, and values ranged from 0 (no immunoreaction) to 300 (maximum immunoreactivity). The score was obtained by applying the following formula, Hs = 1 × (% light staining) + 2 × (% moderate staining) + 3 × (% strong staining). Scoring was independently performed by 2 of the authors (M. S. and O. M.). Interobserver agreement between these 2 authors was assessed by Cohen κ concordance measure, considering all the intervals in Hs range (0-300) at each 10 units, and with Pearson linear correlation.

Funtion Uniprot: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728, PubMed:19074442). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity).

UniProt ID: P15328

UniProt Link: https://www.uniprot.org/uniprotkb/P15328/entry

Biological function from UniProt: #Transport

Molecular function from UniProt:

Tissue specificity from UniProt: Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium.

Subcellular UniProt: #Cell membrane #Cytoplasmic vesicle #Endosome #Membrane #Secreted

Alternative name from UniProt:

Recommended name: Folate receptor alpha

Gene name from HGNC: FOLR1 (FOLR, FRα)

HPA class: Disease related genes Human disease related genes Metabolic proteins Potential drug targets Transporters

AlphaFold DB: P15328

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P15328

HPA link: https://www.proteinatlas.org/ENSG00000110195-FOLR1

Tissue specificity RNA from HPA: Tissue enhanced (choroid plexus, fallopian tube, lung, salivary gland)

Single cell type specificity Ciliated cells - Cilium & Cell projection (mainly)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Nuclear membrane (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable).

Pathology link: https://www.proteinatlas.org/ENSG00000110195-FOLR1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000110195-FOLR1/pathology/endometrial+cancer

Phenotype ID: 613068

Disease: Neurodegeneration due to cerebral folate transport deficiency (NCFTD)

Note1: The disease is caused by variants affecting the gene represented in this entry

OMIM: 136430;613068;

OMIM link1: https://www.omim.org/entry/613068

OMIM link2: https://www.omim.org/entry/136430;https://www.omim.org/entry/613068;

HGNC ID: HGNC:3791

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3791