Biomarker: cyclin E1

Histology type: endometrioid endometrial carcinoma

Country: Spain

Region: Lleida

Followed up time :

Subgroup 1 name : EEC

Subgroup 1 number: 57

Subgroup 2 name: SC

Subgroup 2 number: 31

Conclusion: We propose an internally and externally validated 9-protein biomarker signature to predict the histologic type of EC (EEC or SC) by IHC. The results also suggest that mixed EEC-SC is molecularly ambiguous.

Sample type : tissue

Sample method: immunohistochemistry (IHC)

Expression pattern : cyclin E1 ≥ 220

Expression elevation: IHC staining was graded semiquantitatively by considering the percentage and intensity of the staining. A histologic score (Hs) was obtained from each sample, and values ranged from 0 (no immunoreaction) to 300 (maximum immunoreactivity). The score was obtained by applying the following formula, Hs = 1 × (% light staining) + 2 × (% moderate staining) + 3 × (% strong staining). Scoring was independently performed by 2 of the authors (M. S. and O. M.). Interobserver agreement between these 2 authors was assessed by Cohen κ concordance measure, considering all the intervals in Hs range (0-300) at each 10 units, and with Pearson linear correlation.

Funtion Uniprot: Essential for the control of the cell cycle at the G1/S (start) transition.

UniProt ID: P24864

UniProt Link: https://www.uniprot.org/uniprotkb/P24864/entry

Biological function from UniProt: #Cell cycle #Cell division

Molecular function from UniProt:

Tissue specificity from UniProt: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells

Subcellular UniProt: #Nucleus

Recommended name: G1/S-specific cyclin-E1

Gene name from HGNC: CCNE1 (CCNE)

HPA class: Cancer-related genes Human disease related genes

AlphaFold DB: P24864

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P24864

HPA link: https://www.proteinatlas.org/ENSG00000105173-CCNE1

Tissue specificity RNA from HPA: Tissue enhanced (bone marrow, placenta)

Tissue expression from HPA: Nuclear and cytoplasmic expression of varying intensity in most tissues. Highest expression in testis and placenta.

Single cell type specificity Cell type enhanced (Proximal enterocytes, Endometrial ciliated cells, Respiratory ciliated cells, Alveolar cells type 1)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Located in Nucleoplasm (Single cell variability, CCD Protein, CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in endometrial cancer (unfavorable), liver cancer (unfavorable) and ovarian cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000105173-CCNE1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000105173-CCNE1/pathology/endometrial+cancer

OMIM: 123837

OMIM link2: https://www.omim.org/entry/123837

HGNC ID: HGNC:1589

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1589