Biomarker: Sam68

Histology type: endometrial carcinoma

Country: China

Region: Shanghai

Followed up time :

Subgroup 1 name : Low or none

Subgroup 1 number: 37

Subgroup 2 name: High

Subgroup 2 number: 58

Conclusion: Sam68 may be useful prognostic marker for EC, and it plays an important role in promoting the cellular proliferation

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : high expression( staining index score≥6)

Expression elevation: Staining proportion was scored as follows: 0, no positive cells; 1, <10 % positive cells; 2, 10 %–35 % positive cells; 3, 35 %–70 % positive cells; and 4, >70 % positive cells. Staining intensity was graded according to the method of mean optical density (MOD): 0, no staining; 1, weak staining (light yellow); 2, moderate staining (yellow brown); and 3, strong staining (brown). Immunoreactivity score (IRS) was calculated as the product of staining intensity score and the percentage of positive cells that range from 0 to 12. Optimized cutoff points for each categorical score were determined using log-ranked statistics. This scoring is based on methods previously described that a staining index score≥6 was considered high Sam68 expression, whereas a staining index score ≤4 was considered low Sam68 expression and score=0 was considered negative Sam68 expression.

Statictics: cutoff<50;cutoff>50

Cohort age: 22;73

Subgroup 1 age: 8;14

Subgroup 2 age: 29;44

Funtion Uniprot: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824). RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner (By similarity). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (PubMed:17371836, PubMed:20186123). Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity).Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.

UniProt ID: Q07666

UniProt Link: https://www.uniprot.org/uniprotkb/Q07666/entry

Biological function from UniProt: #Cell cycle #mRNA processing #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver.

Subcellular UniProt: #Cytoplasm #Membrane #Nucleus

Alternative name from UniProt:

Recommended name: KH domain-containing, RNA-binding, signal transduction-associated protein 1

Gene name from HGNC: KHDRBS1 (FLJ34027, p62, Sam68)

AlphaFold DB: Q07666

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q07666

Developmental stage: Isoform 3 is only expressed in growth-arrested cells.

HPA link: https://www.proteinatlas.org/ENSG00000121774-KHDRBS1

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous nuclear expression.

Single cell type specificity Non-specific - Basic cellular processes (mainly)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Nucleoplasm

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable) and renal cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000121774-KHDRBS1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000121774-KHDRBS1/pathology/endometrial+cancer

OMIM: 602489

OMIM link2: https://www.omim.org/entry/602489

HGNC ID: HGNC:18116

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:18116