Biomarker: Enolase-1

Histology type: endometrial carcinoma

Country: China

Region: Guangdong

Study type: prospective study

Followed up time :

Subgroup 1 name : Low expression

Subgroup 1 number: 48

Subgroup 2 name: High expression

Subgroup 2 number: 52

Conclusion: Patients with higher ENO1 expression had a markedly shorter overall survival than patients with low ENO1 expression.

Sample type : tissue

Sample method: immunohistochemical staining

Expression elevation: The staining results were defined based on the percentage of positive nuclei staining. Tumors with 80% or more nuclear staining were regarded as high expression, and less than 80% were regarded as low expression.

Statictics: cutoff<50;cutoff>50

Cohort age: 34;66

Subgroup 1 age: 15;33

Subgroup 2 age: 19;33

Funtion Uniprot: Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209). MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

UniProt ID: P06733

UniProt Link: https://www.uniprot.org/uniprotkb/P06733/entry

Biological function from UniProt: #Glycolysis #Plasminogen activation #Transcription #Transcription regulation

Molecular function from UniProt:

Tissue specificity from UniProt: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Subcellular UniProt: #Cell membrane #Cytoplasm #Membrane #Nucleus

Alternative name from UniProt:

Miscellaneous: Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

Catalytic activity: (2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate

Recommended name: Alpha-enolase

Gene name from HGNC: ENO1 (ENO1L1, MBP-1, MPB1, PPH)

HPA class: Cancer-related genes Enzymes Metabolic proteins Plasma proteins

AlphaFold DB: P06733

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P06733

Induction: Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).

Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

HPA link: https://www.proteinatlas.org/ENSG00000074800-ENO1

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic and nuclear expression.

Single cell type specificity Cell type enhanced (Muller glia cells, Ductal cells, Extravillous trophoblasts)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Plasma membrane, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable) and glioma (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000074800-ENO1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000074800-ENO1/pathology/endometrial+cancer

Expression figure legend: ENO1 protein was expressed in the cytoplasm of NE, endometrial atypical hyperplasia (EAH) and EC samples (original magnification: 400X)

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558174/figure/F8/

Survival figure legend: Expression of ENO1 protein predicts EC patients' overall survival.

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558174/figure/F8/

OMIM: 172430

OMIM link2: https://www.omim.org/entry/172430

HGNC ID: HGNC:3350

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:3350