Biomarker: CDC20

Histology type: endometrial carcinoma

Stage: advanced stage

Country: Egypt

Region: El-Minia

Followed up time :

Conclusion: CDC20 expression may be useful as a biomarker of tumor prognosis and as a therapeutic target of human cancer.

Sample type : tissue

Sample method: immunohistochemistry 

Expression pattern : overexpression(>cutoff )

Expression elevation: The percent of positive cells was scored as 0=≤10 %, 1=>10 to ≤25 %, 2=>25 to ≤50 %, 3=>50 to≤75 %, and 4=>75 %. The intensity of staining was scored as 0=negative, 1=weak, 2=moderate, and 3=strong. The two scores were then multiplied to calculate the final score. The cutoff points for CDC20 immunostaining were the median of CDC20 expression in each normal/non-neoplastic tissue. CDC20 expression was considered negative/low and high expression according to these cutoff points.

Funtion Uniprot: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

UniProt ID: Q12834

UniProt Link: https://www.uniprot.org/uniprotkb/Q12834/entry

Biological function from UniProt: #Cell cycle #Cell division #Differentiation #Mitosis #Neurogenesis #Ubl conjugation pathway

Molecular function from UniProt:

Tissue specificity from UniProt: Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.

Subcellular UniProt: #Cytoplasm #Cytoskeleton

Alternative name from UniProt:

Recommended name: Cell division cycle protein 20 homolog

Gene name from HGNC: CDC20 (CDC20A, p55CDC)

HPA class: Cancer-related genes Plasma proteins Transporters

AlphaFold DB: Q12834

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q12834

HPA link: https://www.proteinatlas.org/ENSG00000117399-CDC20

Tissue specificity RNA from HPA: Tissue enhanced (bone marrow, lymphoid tissue, testis)

Tissue expression from HPA: Cytoplasmic expression in proliferating cells.

Single cell type specificity Cell type enhanced (Extravillous trophoblasts, Early spermatids, Spermatocytes, Erythroid cells, Cytotrophoblasts)

Immune cell specificity: Group enriched (T-reg, NK-cell, memory B-cell, memory CD8 T-cell, memory CD4 T-cell)

Subcellular summary HPA Located in Nucleoplasm, Cytosol (Single cell variability, CCD Protein, CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable), liver cancer (unfavorable) and pancreatic cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000117399-CDC20/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000117399-CDC20/pathology/endometrial+cancer

OMIM: 603618

OMIM link2: https://www.omim.org/entry/603618

HGNC ID: HGNC:1723

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:1723