Biomarker: SPAG9

Histology type: endometrioid endometrial carcinoma

Stage: Grade 1

Country: China

Region: Shandong

Study type: prospective study

Followed up time :

Subgroup 1 name : Grade 1 Endometrioid Adenocarcinoma

Subgroup 1 number: 8

Subgroup 2 name: Endometrial Hyperplasia

Subgroup 2 number: 19

Conclusion: SPAG9 may be associated with the efficacy of progestin treatment in CAEH and grade 1 EA. It may help to distinguish CAEH from grade 1 EA and serve as a new prognostic marker of CAEH or grade 1 EA treated with progestin.

Sample type : tissue

Sample method: immunohistochemistry

Expression elevation: Evaluation of immunohistochemistry was scored by extent (the positive frequency of endometrial gland) and its intensity. The staining intensity was estimated on a four-step scale (0 = no staining; 1 = weak staining; 2 = moderate staining; 3 = strong staining).The fraction of stained gland was scored based on the following criteria: score 0 (0–10% positive endometrial gland), score 1 (11–50% positive endometrial gland), score 2 (51–80% positive endometrial gland), score 3 (>80% positive endometrial gland). The final score were the product of the above two scores.

Funtion Uniprot: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity).

UniProt ID: O60271

UniProt Link: https://www.uniprot.org/uniprotkb/O60271/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed only in testis on the round spermatids of stage I, II and II. Absent in spermatogonia and spermatocyte. Isoform 4 Expressed in testis and in acute myeloid leukemia (AML) patients. Isoform 3 Expressed in testis.

Subcellular UniProt: #Cytoplasm #Cytoplasmic vesicle #Lysosome #Membrane

Alternative name from UniProt:

Activity regulation: Isoform 5 May play a role in spermatozoa-egg-interaction.

Recommended name: C-Jun-amino-terminal kinase-interacting protein 4

Gene name from HGNC: SPAG9 (CT89, FLJ13450, FLJ14006, FLJ26141, FLJ34602, HLC4, HSS, JIP-4, JIP4, JLP, KIAA0516, MGC117291, MGC14967, MGC74461, PHET, PIG6, SYD1)

HPA class: Plasma proteins

AlphaFold DB: O60271

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/O60271

HPA link: https://www.proteinatlas.org/ENSG00000008294-SPAG9

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic expression.

Single cell type specificity Low cell type specificity

Immune cell specificity: Group enriched (neutrophil, basophil)

Subcellular summary HPA Located in Centriolar satellite, Cytosol

Cancer prognostic summary HPA Prognostic marker in urothelial cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000008294-SPAG9/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000008294-SPAG9/pathology/endometrial+cancer

OMIM: 605430

OMIM link2: https://www.omim.org/entry/605430

HGNC ID: HGNC:14524

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:14524