Biomarker: ANXA2

Histology type: endometrial carcinoma

Country: China

Region: Shengjing

Followed up time :

Subgroup 1 name : positive expression

Subgroup 1 number: 80

Subgroup 2 name: High

Subgroup 2 number: 55

Conclusion: Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma, and ANXA2 was an independent risk factor for poor prognosis. The expression of ANXA2 and HE4 can affect each other.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : overexpresses(0 to 2 is considered (−); 3 to 4, (+); 5 to 8, (++); and 9 to 12, (+++))

Expression elevation: Consider a positive result if there are buffy granules in the cell membrane and cytoplasm. According to the chromatosis intensity, no pigmentation, light yellow, buffy, and brown are scored 0, 1, 2, and 3, respectively. We choose 5 high-power fields in series from each slice, then score them and take the mean percentage of the chromatosis cells: chromatosis cells that account less than 5 % are 0, 5 % to 25 %: 1, 26 % to 50 %: 2, 51 % to 75 %: 3, and greater than 75 %: 4. Multiply these 2 numbers: 0 to 2 is considered (−); 3 to 4, (+); 5 to 8, (++); and 9 to 12, (+++).

Statictics: cutoff<59;cutoff>59

Cohort age: 42;42

Subgroup 1 age: 39;41

Subgroup 2 age: 26;29

Funtion Uniprot: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:24808179, PubMed:22848640).

UniProt ID: P07355

UniProt Link: https://www.uniprot.org/uniprotkb/P07355/entry

Biological function from UniProt: #Host-virus interaction

Molecular function from UniProt:

Subcellular UniProt: #Basement membrane #Extracellular matrix #Secreted

Alternative name from UniProt:

Miscellaneous: It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Recommended name: Annexin A2

Gene name from HGNC: ANXA2 (ANX2, ANX2L4, CAL1H, LIP2, LPC2D)

HPA class: Cancer-related genes FDA approved drug targets Plasma proteins Transporters

AlphaFold DB: P07355

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P07355

HPA link: https://www.proteinatlas.org/ENSG00000182718-ANXA2

Tissue specificity RNA from HPA: Tissue enhanced (esophagus)

Tissue expression from HPA: Ubiquitous membranous and extracellular expression.

Single cell type specificity Cell type enhanced (Basal respiratory cells, Pancreatic endocrine cells, Prostatic glandular cells, Urothelial cells)

Immune cell specificity: Group enriched (basophil, myeloid DC, classical monocyte, non-classical monocyte, intermediate monocyte, T-reg)

Subcellular summary HPA Located in Plasma membrane, Cytosol

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable), pancreatic cancer (unfavorable), liver cancer (unfavorable), urothelial cancer (unfavorable), endometrial cancer (favorable) and lung cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000182718-ANXA2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000182718-ANXA2/pathology/endometrial+cancer

Expression figure legend: Expression and co-localization of ANXA2-HE4 in endometrial tissues.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/figure/Fig3/

Survival figure legend: Univariate Kaplan–Meier analysis of endometrial cancinoma

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/figure/Fig4/

OMIM: 151740

OMIM link2: https://www.uniprot.org/uniprotkb/P07355/external-links

HGNC ID: HGNC:537

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:537