Biomarker: HE4

Histology type: endometrial carcinoma

Country: China

Region: Shengjing

Followed up time :

Subgroup 1 name : Positive

Subgroup 1 number: 88

Subgroup 2 name: Strong positive

Subgroup 2 number: 33

Conclusion: Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma, and ANXA2 was an independent risk factor for poor prognosis. The expression of ANXA2 and HE4 can affect each other.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : co-expression of ANXA2 and HE4

Expression elevation: Consider a positive result if there are buffy granules in the cell membrane and cytoplasm. According to the chromatosis intensity, no pigmentation, light yellow, buffy, and brown are scored 0, 1, 2, and 3, respectively. We choose 5 high-power fields in series from each slice, then score them and take the mean percentage of the chromatosis cells: chromatosis cells that account less than 5 % are 0, 5 % to 25 %: 1, 26 % to 50 %: 2, 51 % to 75 %: 3, and greater than 75 %: 4. Multiply these 2 numbers: 0 to 2 is considered (−); 3 to 4, (+); 5 to 8, (++); and 9 to 12, (+++).

Statictics: Mean (SD);Range

Cohort age: 58.09;31-79

Funtion Uniprot: Broad range protease inhibitor.

UniProt ID: Q14508

UniProt Link: https://www.uniprot.org/uniprotkb/Q14508/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

Subcellular UniProt: #Secreted

Alternative name from UniProt:

Recommended name: WAP four-disulfide core domain protein 2

Gene name from HGNC: WFDC2 (dJ461P17.6, EDDM4, HE4, WAP5)

HPA class: Cancer-related genes

AlphaFold DB: Q14508

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q14508

HPA link: https://www.proteinatlas.org/ENSG00000101443-WFDC2

Tissue specificity RNA from HPA: Tissue enhanced (cervix, salivary gland)

Tissue expression from HPA: Cytoplasmic expression in selected tissues.

Single cell type specificity Cell type enhanced (Club cells, Glandular and luminal cells, Basal respiratory cells, Urothelial cells, Endometrial ciliated cells, Respiratory ciliated cells, Collecting duct cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Secreted

Cancer prognostic summary HPA Prognostic marker in glioma (unfavorable) and renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000101443-WFDC2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000101443-WFDC2//pathology/endometrial+cancer

Expression figure legend: Expression and co-localization of ANXA2-HE4 in endometrial tissues.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/figure/Fig3/

Survival figure legend: Univariate Kaplan–Meier analysis of endometrial cancinoma

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4567805/figure/Fig4/

OMIM: 617548

OMIM link2: https://www.omim.org/entry/617548

HGNC ID: HGNC:15939

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:15939