Biomarker: HE4

Histology type: endometrial carcinoma

Country: China

Region: Liaoning

Followed up time :

Subgroup 1 name : Positive

Subgroup 1 number: 88

Subgroup 2 name: Strong positive

Subgroup 2 number: 33

Conclusion: Endometrial cancer patients with high HE4 expression have a poor prognosis.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: Immunohistochemical staining results, brown-stained granules on the cell membrane and cytoplasm, were regarded as positive. Based on the strength of color, uncolored, light yellow, yellowish brown, and brown were scored as 0, 1, 2, and 3, respectively. The percentage of stained cells in the field of view was calculated as follows: five consecutive high-powered fields in each section were observed under a 400x optical microscope, and then the scores were averaged. The proportion of positive cells <5% was recorded as 0, 5%–25% as 1, 21%–50% as 2, 51%–75% as 3, and >75% as 4. The final score was equal to the multiplication of the two scores: 0–2 as negative (−), 3-4 as weakly positive (+), and 5–12 as strongly positive (2+/3+) expression.

Statictics: Mean (SD);Range

Cohort age: 58.09;31-79

Funtion Uniprot: Broad range protease inhibitor.

UniProt ID: Q14508

UniProt Link: https://www.uniprot.org/uniprotkb/Q14508/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

Subcellular UniProt: #Secreted

Alternative name from UniProt:

Recommended name: WAP four-disulfide core domain protein 2

Gene name from HGNC: WFDC2 (dJ461P17.6, EDDM4, HE4, WAP5)

HPA class: Cancer-related genes

AlphaFold DB: Q14508

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q14508

HPA link: https://www.proteinatlas.org/ENSG00000101443-WFDC2

Tissue specificity RNA from HPA: Tissue enhanced (cervix, salivary gland)

Tissue expression from HPA: Cytoplasmic expression in selected tissues.

Single cell type specificity Cell type enhanced (Club cells, Glandular and luminal cells, Basal respiratory cells, Urothelial cells, Endometrial ciliated cells, Respiratory ciliated cells, Collecting duct cells)

Immune cell specificity: Not detected in immune cells

Subcellular summary HPA Secreted

Cancer prognostic summary HPA Prognostic marker in glioma (unfavorable) and renal cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000101443-WFDC2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000101443-WFDC2//pathology/endometrial+cancer

Expression figure legend: Immunohistochemical micrographs of HE4 in different endometrial tissues (200x).

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619810/figure/fig1/

Survival figure legend: survival rates

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619810/figure/fig2/

OMIM: 617548

OMIM link2: https://www.omim.org/entry/617548

HGNC ID: HGNC:15939

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:15939