Biomarker: TOP2A

Histology type: endometrial carcinoma

Stage: moderate- to high-risk

Country: Japan

Region: Nara

Study type: retrospective study

Followed up time :

Subgroup 1 name : Positive

Subgroup 1 number: 7

Subgroup 2 name: Negative

Subgroup 2 number: 49

Conclusion: Patients with TOP2A overexpression have a worse prognosis compared with those with TOP2A nonexpression, and TOP2A may be a useful biomarker in patients receiving adjuvant taxane-platinum regimens with moderate- to high-risk endometrial cancer.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : overexpression

Expression elevation: TOP2A staining intensity was scored as 0 to 3+ based on the percentage of positive cells: 0 (no staining), 1+ (<10%), 2+ (10%-50%), or 3+ (>50%). A score of 3+ was defined as TOP2A positive. Two independent pathologists blinded to the clinical data and each other’s opinion scored the expression based on the staining intensity.

Statictics: Median;Range

Cohort age: 61;41-77

Funtion Uniprot: Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

UniProt ID: P11388

UniProt Link: https://www.uniprot.org/uniprotkb/P11388/entry

Biological function from UniProt: #Biological rhythms

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056).

Subcellular UniProt: #Cytoplasm #Nucleus

Alternative name from UniProt:

Miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Activity regulation: Specifically inhibited by the intercalating agent amsacrine.

Recommended name: DNA topoisomerase 2-alpha

Gene name from HGNC: TOP2A (TOP2)

HPA class: Cancer-related genes Enzymes FDA approved drug targets Plasma proteins

AlphaFold DB: P11388

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P11388

HPA link: https://www.proteinatlas.org/ENSG00000131747-TOP2A

Tissue specificity RNA from HPA: Tissue enhanced (lymphoid tissue, testis)

Tissue expression from HPA: Nuclear expression mainly observed in highly proliferative cells.

Single cell type specificity Cell type enhanced (Spermatocytes, Spermatogonia, Erythroid cells, Granulosa cells)

Immune cell specificity: Immune cell enhanced (T-reg)

Subcellular summary HPA Located in Nucleoplasm, Nucleoli (Single cell variability, CCD Protein, CCD Transcript)

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable), liver cancer (unfavorable), pancreatic cancer (unfavorable) and lung cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000131747-TOP2A/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000131747-TOP2A/pathology/endometrial+cancer

OMIM: 126430

OMIM link2: https://www.omim.org/entry/126430

HGNC ID: HGNC:11989

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:11989