Biomarker: GAL-3

Histology type: endometrial carcinoma

Country: Greece

Region: Thessaloniki

Followed up time :

Conclusion: In conclusion, we found that the two proteins follow the same expression pattern and that their co-expression is strongly correlated with poor prognosis and short survival.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : co-expression of galectin-3 and CRIP-1

Expression elevation: The average labeling index of GAL-3 and CRIP-1 was assessed according to the proportion of positive cells, after scanning the entire section of the specimen. Sections were graded as negative (0) for <10 % of stained cells, low (1) for 10–30 % of stained cells, moderate (2) for 30–70 % of stained cells and high (3) for >70 % of stained cells. Staining intensity was equal between different samples and areas of the tissue sections, and therefore, it was not included in the scoring

Statictics: Mean (SD)

Cohort age: 60.02 ± 10.61

Funtion Uniprot: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes

UniProt ID: P17931

UniProt Link: https://www.uniprot.org/uniprotkb/P17931/entry#function

Molecular function from UniProt:

Tissue specificity from UniProt: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

Subcellular UniProt: #Cytoplasm #Nucleus #Secreted #Spliceosome

Alternative name from UniProt:

Recommended name: Galectin-3

Gene name from HGNC: LGALS3 (GALIG, LGALS2, MAC-2)

HPA class: Cancer-related genes Plasma proteins

AlphaFold DB: P17931

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P17931

HPA link: https://www.proteinatlas.org/ENSG00000131981-LGALS3

Tissue specificity RNA from HPA: Tissue enhanced (intestine)

Tissue expression from HPA: Cytoplasmic expression in most tissues.

Single cell type specificity Cell type enhanced (Proximal enterocytes, Distal enterocytes, Paneth cells)

Immune cell specificity: Group enriched (classical monocyte, intermediate monocyte, non-classical monocyte, myeloid DC, T-reg)

Subcellular summary HPA Located in Nucleoplasm, Plasma membrane, Cytosol (Single cell variability)

Cancer prognostic summary HPA Gene product is not prognostic

Pathology link: https://www.proteinatlas.org/ENSG00000131981-LGALS3/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000131981-LGALS3/pathology/endometrial+cancer

Expression figure legend: GAL-3-positive immunohistochemistry stain X200. GAL-3-negative immunohistochemistry stain X200 (inset) $CRIP-1-positive immunohistochemistry stain X200. CRIP-1-negative immunohistochemistry stain X200

Expression figure link: https://link.springer.com/article/10.1007/s12032-015-0723-7/figures/2 $https://link.springer.com/article/10.1007/s12032-015-0723-7/figures/3

Survival figure legend: Co-expression of galectin-3 and CRIP-1 in endometrial cancer: prognostic value and patient survival

Survival curve link: https://link.springer.com/article/10.1007/s12032-015-0723-7/figures/5

OMIM: 153619

OMIM link2: https://www.uniprot.org/uniprotkb/P17931/external-links

HGNC ID: HGNC:6563

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:6563