Biomarker: NUCB2

Histology type: endometrial carcinoma

Country: Japan

Region: Sendai

Study type: retrospective study

Followed up time :

Subgroup 1 name : Negative

Subgroup 1 number: 68

Subgroup 2 name: Positive

Subgroup 2 number: 19

Conclusion: Immunohistochemical NUCB2 status may therefore serve as a potent biomarker for endometrial carcinomas.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : positive

Expression elevation: NUCB2 immunoreactivity was detected in the cytoplasm of carcinoma cells and also non-neoplastic glands, and the cases that had more than 10% positive carcinoma cells were considered positive for NUCB2 immunoreactivity.

Funtion description: Subsequent in vitro studies demonstrated that NUCB2 significantly promoted cell proliferation and migration property in Ishikawa and Sawano cells.

Funtion Uniprot: Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (By similarity).; Nesfatin-1 Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

UniProt ID: P80303

UniProt Link: https://www.uniprot.org/uniprotkb/P80303/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Predominantly expressed in spleen, testis and normal stomach.

Subcellular UniProt: #Cytoplasm #Endoplasmic reticulum #Golgi apparatus #Membrane #Nucleus #Secreted

Alternative name from UniProt:

Cleaved: Cleaved into 1 chains :Nesfatin-1

Miscellaneous: NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

Recommended name: Nucleobindin-2

Gene name from HGNC: NUCB2 (NEFA)

HPA class: Cancer-related genes Plasma proteins

AlphaFold DB: P80303

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P80303

HPA link: https://www.proteinatlas.org/ENSG00000070081-NUCB2

Tissue specificity RNA from HPA: Tissue enhanced (pancreas, salivary gland)

Tissue expression from HPA: Cytoplasmic expression in most tissues, including immune cells.

Single cell type specificity Cell type enhanced (Syncytiotrophoblasts, Early spermatids, Respiratory ciliated cells, Late spermatids)

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Golgi apparatus

Cancer prognostic summary HPA Prognostic marker in endometrial cancer (favorable), renal cancer (unfavorable) and ovarian cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000070081-NUCB2/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000070081-NUCB2/pathology/endometrial+cancer

OMIM: 608020

OMIM link2: https://www.omim.org/entry/608020

HGNC ID: HGNC:8044

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:8044