Biomarker: CD169

Histology type: endometrial carcinoma

Country: Japan

Region: Kumamoto

Followed up time :

Subgroup 1 name : <350

Subgroup 1 number: 36

Subgroup 2 name: >=350

Subgroup 2 number: 35

Conclusion: CD169(+) macrophages in RLN might represent a useful marker for assessing clinical prognoses and monitoring anti-tumor immunity in patients with EC.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : CD169(+) macrophages in RLN

Expression elevation: Calculate the number of positive cells per unit area, we measured the areas (mm2) using the ImageJ software program (US National Institute of Health, Bethesda, MD, USA).

Statictics: cutoff<60;cutoff>60

Cohort age: 41;38

Subgroup 1 age: 20;19

Subgroup 2 age: 21;19

Funtion Uniprot: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis

UniProt ID: Q9BZZ2

UniProt Link: https://www.uniprot.org/uniprotkb/Q9BZZ2/entry

Biological function from UniProt: #Lectin

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

Subcellular UniProt: #Cell membrane #Membrane #Secreted

Alternative name from UniProt:

Recommended name: Sialoadhesin

Gene name from HGNC: This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]

CD antigen name: CD169

HPA class: CD markers

AlphaFold DB: Q9BZZ2

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/Q9BZZ2

HPA link: https://www.proteinatlas.org/ENSG00000088827-SIGLEC1

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Membranous expression in macrophages in several tissues.

Single cell type specificity Group enriched (Kupffer cells, Hofbauer cells, Macrophages)

Immune cell specificity: Immune cell enhanced (classical monocyte)

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable) and testis cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000088827-SIGLEC1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000088827-SIGLEC1/pathology/endometrial+cancer

Expression figure legend: Immunostaining of sinus macrophages in the regional lymph node (RLN) of the endometrial carcinoma (EC) patients.$ Interactions between CD169+ macrophages in the regional lymph node (RLN) and CD8+ T cells or CD57+ NK cells in endometrial carcinoma (EC)

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968600/figure/cas12929-fig-0001/$ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968600/figure/cas12929-fig-0003/

Survival figure legend: Kaplan–Meier survival curves for 79 endometrial carcinoma (EC) patients as related to the number of CD169+ macrophages, CD169+‐to‐CD68+ macrophage ratio, and number of CD68+ macrophages in regional lymph node (RLN). LN, lymph node.

Survival curve link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968600/figure/cas12929-fig-0002/

OMIM: 600751

OMIM link2: https://www.omim.org/entry/600751

HGNC ID: HGNC:11127

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:11127