Biomarker: USP14

Histology type: endometrial carcinoma

Country: USA

Region: Minnesota

Study type: retrospective study

Followed up time :

Subgroup 1 name : Not Recured

Subgroup 1 number: 88

Subgroup 2 name: Recurred

Subgroup 2 number: 19

Conclusion: USP14 is a predictor of recurrence in endometrial cancer and a molecular target for endometrial cancer treatment.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression

Expression elevation: The staining intensity was rated as follows: 0 = no staining, 1+ = weak intensity, 2+ = moderate intensity and 3+ = high intensity.

Statictics: Mean (SD)

Cohort age: 60.6±9.6

Funtion Uniprot: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593).

UniProt ID: P54578

UniProt Link: https://www.uniprot.org/uniprotkb/P54578/entry

Biological function from UniProt: Immunity, Innate immunity, Ubl conjugation pathway

Molecular function from UniProt:

Subcellular UniProt: #Cell membrane #Cytoplasm #Membrane #Proteasome

Alternative name from UniProt:

Caution: Was originally thought to be a guanine tRNA-ribosyltransferase.

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Recommended name: Ubiquitin carboxyl-terminal hydrolase 14

Gene name from HGNC: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

HPA class: Enzymes Metabolic proteins Plasma proteins

AlphaFold DB: P54578

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P54578

HPA link: https://www.proteinatlas.org/ENSG00000101557-USP14

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Ubiquitous cytoplasmic expression.

Single cell type specificity Low cell type specificity

Immune cell specificity: Low immune cell specificity

Subcellular summary HPA Located in Plasma membrane, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in liver cancer (unfavorable), head and neck cancer (unfavorable) and ovarian cancer (favorable)

Pathology link: https://www.proteinatlas.org/ENSG00000101557-USP14/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000101557-USP14/pathology

Expression figure legend: USP14 is overexpressed at diagnosis of endometrial adenocarcinoma among patients who eventually recur

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058731/figure/F1/

OMIM: 607274

OMIM link2: https://www.omim.org/entry/607274

HGNC ID: HGNC:12612

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:12612