Biomarker: IFITM1

Histology type: endometrioid endometrial carcinoma

Country: Canada

Region: Ottaw

Study type: retrospective study

Followed up time :

Conclusion: IFITM1 expression is highly predictive of the absence of invasion and may be valuable in cases in which determining myoinvasion has staging implications.IFITM1 had 71.4% sensitivity and 100% specificity in detecting stroma surrounding endometrioid adenocarcinoma, hence excluding myoinvasion.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : expression(total score of 3 to 6 was classified as positive)

Expression elevation: The mesenchyma surrounding adenocarcinoma glands was scored as follows: 1. Intensity: 0 = absent staining, 1 = weak staining, 2 = moderate staining, 3 = strong staining 2. Distribution: 0 = absent staining, 1 = less than 50% of the focus circumference, 2 = 50% to 75% of circumference, 3 = more than 75% of the circumference A total score was calculated by adding intensity and distribution scores for each area (ranging from 0 to 6). For grouping purposes, a total score of 0 to 2 was considered negative, and a total score of 3 to 6 was classified as positive.

Statictics: Mean ;Range

Cohort age: 62.5;38-79

Funtion Uniprot: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronaviruses (SARS-CoV and SARS-CoV-2), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) (PubMed:26354436, PubMed:33270927). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV and SARS-CoV-2 S protein-mediated viral entry. Also implicated in cell adhesion and control of cell growth and migration (PubMed:33270927). Inhibits SARS-CoV-2 S protein-mediated syncytia formation (PubMed:33051876). Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Acts as a positive regulator of osteoblast differentiation. In hepatocytes, IFITM proteins act in a coordinated manner to restrict HCV infection by targeting the endocytosed HCV virion for lysosomal degradation (PubMed:26354436). IFITM2 and IFITM3 display anti-HCV activity that may complement the anti-HCV activity of IFITM1 by inhibiting the late stages of HCV entry, possibly in a coordinated manner by trapping the virion in the endosomal pathway and targeting it for degradation at the lysosome (PubMed:26354436).

UniProt ID: P13164

UniProt Link: https://www.uniprot.org/uniprotkb/P13164/entry

Biological function from UniProt: Antiviral defense, Immunity, Innate immunity, Osteogenesis

Molecular function from UniProt:

Tissue specificity from UniProt: Bone (at protein level). Levels greatly elevated in colon cancer, cervical cancer, esophageal cancer and ovarian cancer. Expressed in glioma cell lines.

Subcellular UniProt: #Cell membrane #Lysosome #Membrane

Alternative name from UniProt:

Recommended name: Interferon-induced transmembrane protein 1

Gene name from HGNC: IFITM1 (9-27, CD225, DSPA2a, IFI17)

CD antigen name: CD225

HPA class: CD markers Plasma proteins Transporters

AlphaFold DB: P13164

AlphaFold Link: https://alphafold.ebi.ac.uk/entry/P13164

Induction: By IFN-alpha and IFNG/IFN-gamma.

HPA link: https://www.proteinatlas.org/ENSG00000185885-IFITM1

Tissue specificity RNA from HPA: Low tissue specificity

Tissue expression from HPA: Distinct membranous expression in epididymis, also expressed in endothelial cells and in lymphoid tissue.

Single cell type specificity Cell type enhanced (Basal squamous epithelial cells, T-cells, Endothelial cells, Leydig cells, Sertoli cells)

Immune cell specificity: Group enriched (NK-cell, memory CD4 T-cell, memory CD8 T-cell, naive CD8 T-cell, naive CD4 T-cell, gdT-cell, neutrophil, MAIT T-cell, T-reg, eosinophil)

Subcellular summary HPA Located in Golgi apparatus, Plasma membrane, Cytosol (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in renal cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000185885-IFITM1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000185885-IFITM1/pathology/endometrial+cancer

Expression figure legend: Absence of interferon-induced transmembrane protein 1 (IFITM1) expression in the mesenchyma surrounding adenocarcinoma. Endothelium in interspersed capillaries is positive for IFITM1, which serves as an internal positive control.

Expression figure link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109682/figure/aqw021-F2/

OMIM: 604456

OMIM link2: https://www.omim.org/entry/604456

HGNC ID: HGNC:5412

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:5412