Biomarker: Asparaginase-like protein 1

Histology type: endometrial carcinoma

Country: Norway

Region: Bergen

Study type: prospectively study

Followed up time :

Subgroup 1 name : High

Subgroup 1 number: 539

Subgroup 2 name: Low

Subgroup 2 number: 84

Conclusion: Asparaginase-like protein 1 is an independent prognostic marker in primary endometrial cancer, and is frequently lost in metastatic lesions.

Sample type : tissue

Sample method: immunohistochemistry

Expression pattern : loss expression

Expression elevation: A staining index (SI) was calculated for each patient based on all three cylinders by multiplying staining intensity (range 0–3) and area of positive stained tumor cells (1 ≤ 10%, 2 = 10–50%, 3 ≥ 50%) as previously reported. The upper three quartiles were combined based on similarities in survival, and defined as “ASRGL1 high”. The lower quartile was defined as “ASRGL1 low”, corresponding to SI: 0–2 (HPA029725) or SI:0–1 (AMAb90907).

Statictics: cutoff<66;cutoff>66

Cohort age: 405;377

Subgroup 1 age: 341;275

Subgroup 2 age: 64;102

Funtion Uniprot: Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.

UniProt ID: Q7L266

UniProt Link: https://www.uniprot.org/uniprotkb/Q7L266/entry

Molecular function from UniProt:

Tissue specificity from UniProt: Expressed in brain, kidney, testis and tissues of the gastrointestinal tract. Present in sperm (at protein level). Over-expressed in uterine, mammary, prostatic and ovarian carcinoma.

Subcellular UniProt: #Cytoplasm

Alternative name from UniProt:

Cleaved: Cleaved into 2 chains:Isoaspartyl peptidase/L-asparaginase alpha chain Isoaspartyl peptidase/L-asparaginase beta chain

Catalytic activity: H2O + L-asparagine = L-aspartate + NH4+

Gene name from HGNC: ASRGL1 (ALP, ALP1, FLJ22316)

Induction: By 5-alpha-di-hydrotestosterone and progesterone.

HPA link: https://www.proteinatlas.org/ENSG00000162174-ASRGL1

Tissue specificity RNA from HPA: Tissue enhanced (brain, cervix, testis)

Tissue expression from HPA: Cytoplasmic expression most abundant in seminiferous ducts and glandular cells of female genital tract.

Subcellular summary HPA Located in Nucleoplasm, Microtubules, Cytokinetic bridge (Single cell variability)

Cancer prognostic summary HPA Prognostic marker in renal cancer (favorable), endometrial cancer (favorable), colorectal cancer (favorable) and liver cancer (unfavorable)

Pathology link: https://www.proteinatlas.org/ENSG00000162174-ASRGL1/pathology

Pathology endo: https://www.proteinatlas.org/ENSG00000162174-ASRGL1/pathology/endometrial+cancer

HGNC ID: HGNC:16448

HGNC link: https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:16448